CAJ | 학술논문

目的探讨低密度脂蛋白受体基因(LDLR)PvuⅡ多态性对健康青年人血脂比值的影响及在高糖低脂(HC/LF)膳食诱导的血脂比值变化中的作用.方法 56名[(22.89±1.80)岁]健康志愿者,给予7 d平衡膳食和6 d HC/LF膳食.分别于第1 d、第8 d及第14 d取空腹静脉血,测定血脂水平,计算甘油三酯/高密度脂蛋白-胆固醇(TG/HDL-C)、log(TG/HDL-C)、总胆固醇(TC)/HDL-C、低密度脂蛋白-胆固醇(LDL-C)/HDL-C值.分离基因组DNA,聚合酶链反应-限制性酶切法分析LDLR PvuⅡ位点多态性.分析不同基因型间血脂比值差异.结果不同基因型受试者血脂比值的基础值无明显差异.HC/LF膳食前后,无论是在受试人群整体还是男女分组分析,不同基因型之间血脂比值均无明显差异.与HC/LF膳食前相比,在受试人群整体,不同基因型受试者LDL-C/HDL-C和TC/HDL-C均降低(P<0.05).男女分组分析发现,与HC/LF膳食前相比,HC/LF膳食后TC/HDL-C在男性和女性均下降(P<0.05),但不受LDLR PvuⅡ多态性影响;TG/HDL-C和log(TG/HDL-C)仅在女性升高(P<0.05),也不受LDLR PvuⅡ多态性影响;而LDL-C/HDL-C在女性P1P1基因型、P2携带者和男性P1P1基因型下降(P<0.05).结论 LDLR PvuⅡ位点多态性在健康男性青年与HC/LF膳食诱导的LDL-C/HDL-C改变可能相关联.
목적 탐토저밀도지단백수체기인(LDLR)PvuⅡ다태성대건강청년인혈지비치적영향급재고당저지(HC/LF)선식유도적혈지비치변화중적작용.방법 56명[(22.89±1.80)세]건강지원자,급여7 d평형선식화6 d HC/LF선식.분별우제1 d、제8 d급제14 d취공복정맥혈,측정혈지수평,계산감유삼지/고밀도지단백-담고순(TG/HDL-C)、log(TG/HDL-C)、총담고순(TC)/HDL-C、저밀도지단백-담고순(LDL-C)/HDL-C치.분리기인조DNA,취합매련반응-한제성매절법분석LDLR PvuⅡ위점다태성.분석불동기인형간혈지비치차이.결과 불동기인형수시자혈지비치적기출치무명현차이.HC/LF선식전후,무론시재수시인군정체환시남녀분조분석,불동기인형지간혈지비치균무명현차이.여HC/LF선식전상비,재수시인군정체,불동기인형수시자LDL-C/HDL-C화TC/HDL-C균강저(P<0.05).남녀분조분석발현,여HC/LF선식전상비,HC/LF선식후TC/HDL-C재남성화녀성균하강(P<0.05),단불수LDLR PvuⅡ다태성영향;TG/HDL-C화log(TG/HDL-C)부재녀성승고(P<0.05),야불수LDLR PvuⅡ다태성영향;이LDL-C/HDL-C재녀성P1P1기인형、P2휴대자화남성P1P1기인형하강(P<0.05).결론 LDLR PvuⅡ위점다태성재건강남성청년여HC/LF선식유도적LDL-C/HDL-C개변가능상관련.
Objective To investigate the effects of low density lipoprotein receptor gene (LDLR) PvuⅡ polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat (HC/LF) diet in healthy youth. Methods After a regular diet for 7 days of wash-out, 56 health youth [(22.89±1.80) years old)] were given LF/HC diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The LF/HC diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids were measured on the 1~(st), the 8~(th) and the 14~(th) days. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The polymorphism of LDLR was detected by PCR-RFLP method and its relationship with serum lipid ratios was analyzed. Results No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders. Before or after HC/LF diet, no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately. Compared with those of before HC/LF diet, all the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C (P<0.05). When gender was taken into account, significant increases of TG/HDL-C and log(TG/HDL-C)were found only in females (P<0.05). All the subjects experienced significant decrease of TC/HDL-C regardless of gender and genotype (P<0.05). As for LDL-C/HDL-C, significant decrease was found only in males with P1P1 and female subjects (P<0.05). Conclusion Polymorphism of LDLR PvuⅡ may influence the response of LDL-C/HDL-C to HC/LF diet in males.