CAJ | 학술논문

目的探讨长时间应用大剂量的左旋精氨酸对幼鼠学习记忆功能和神经系统可塑性的影响.方法分别给初断乳的大鼠灌胃大剂量的NO前体左旋精氨酸(L-arginine,L-Arg)[600 mg/(kg·d),L-Arg组]和同样剂量的蒸馏水(对照组),每组各10只,持续3个月.用Morris水迷宫检测大鼠的学习记忆功能,用免疫组化技术和HE染色检测大脑皮质和海马CA1、CA3、DG区的神经元型一氧化氮合酶(nNOS)和c-fos基因的表达以及神经细胞数目的变化.结果实验结果显示大鼠寻台潜伏期的时间和nNOS的表达在L-Arg组明显较对照组升高,但c-fos基因的表达和大脑皮质及海马CA1、CA3、DG区的神经元数目在L-Arg组明显较对照组降低(P<0.05或P<0.01).结论长时间应用大剂量的NO前体L-Arg可抑制幼鼠的学习记忆功能和神经系统的可塑性.
목적 탐토장시간응용대제량적좌선정안산대유서학습기억공능화신경계통가소성적영향.방법 분별급초단유적대서관위대제량적NO전체좌선정안산(L-arginine,L-Arg)[600 mg/(kg·d),L-Arg조]화동양제량적증류수(대조조),매조각10지,지속3개월.용Morris수미궁검측대서적학습기억공능,용면역조화기술화HE염색검측대뇌피질화해마CA1、CA3、DG구적신경원형일양화담합매(nNOS)화c-fos기인적표체이급신경세포수목적 변화.결과 실험결과현시대서심태잠복기적시간화nNOS적표체재L-Arg조명현교대조조승고,단c-fos기인적표체화대뇌피질급해마CA1、CA3、DG구적신경원수목재L-Arg조명현교대조조강저(P<0.05혹P<0.01).결론 장시간응용대제량적NO전체L-Arg가억제유서적학습기억공능화신경계통적가소성.
Objective To study the effects of long-lasting administration of large dose of L-arginine(L-Arg) on the ability of learning and memory and the plasticity of nervous system in junior rats. Methods The weanling rats were commensurate distilled water (control group,n=10) for three months,respectively.The ability of learning and memory was observed by Morris water maze.Then nNOS (neuronal NOS) or c-fos expression with immunocytochemisity and the number of neuronal cells with HE staining in cerebral cortex and CA1,CA3,DG of hippocampus were detected. Results The latency to find the platform and the expression of nNOS were significantly higher in L-Arg group than that in control group,but the expression of c-fos and the number of neuronal cells in cerebral cortex and CA1,CA3,DG of hippocampus were markedly lower in L-Arg group than that in control group (P<0.05 or P<0.01). Conclusion The results suggest that long-lasting use of L-Arg with large dose can inhibit the ability of learning and memory and the plasticity of nervous system in the developing brain.