CAJ | 학술논문

目的初步分析老年急性髓细胞白血病(AML)患者P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)的表达及其在预后中的意义.方法流式细胞仪活细胞直接或间接免疫荧光法检测12例初治老年AML(M2a4例.其中1例由MDS转化而来;M4a2例;M5a5例;M6a1例)患者骨髓白血病细胞P-gP、MRP、LRP表达情况.分析这些多药耐药蛋白表达与预后的关系.结果 P-gp、MRP、LRP的表达率分别为58.33%、8.33%、50%;P-gp(+)、MRP(+)0,P-gp(+)、LRP(+)33.33%,MRP(+)、LRP(+)0,P-gp(+)、MRP(+)、LRP(+)8.33%,P-gp(-)、MRP(-)、LRP(-)33.33%,其中P-gp、LRP单独和二者共表达频率较高.P-gp(+)者的完全缓解(CR)率与2年总生存率(OS)均显著低于P-gp(-)者(P=0.01),LRP(+)者的CR率与2年OS亦均低于LRP(-)者.结论老年AML患者P-gp、LRP单独及二者共表达频率均较高.P-gp、LRP过度表达是老年AML患者的不良预后因素.
목적 초보분석노년급성수세포백혈병(AML)환자P-당단백(P-gp)、다약내약상관단백(MRP)、폐내약단백(LRP)적표체급기재예후중적의의.방법 류식세포의활세포직접혹간접면역형광법검측12례초치노년AML(M2a4례.기중1례유MDS전화이래;M4a2례;M5a5례;M6a1례)환자골수백혈병세포P-gP、MRP、LRP표체정황.분석저사다약내약단백표체여예후적관계.결과 P-gp、MRP、LRP적표체솔분별위58.33%、8.33%、50%;P-gp(+)、MRP(+)0,P-gp(+)、LRP(+)33.33%,MRP(+)、LRP(+)0,P-gp(+)、MRP(+)、LRP(+)8.33%,P-gp(-)、MRP(-)、LRP(-)33.33%,기중P-gp、LRP단독화이자공표체빈솔교고.P-gp(+)자적완전완해(CR)솔여2년총생존솔(OS)균현저저우P-gp(-)자(P=0.01),LRP(+)자적CR솔여2년OS역균저우LRP(-)자.결론 노년AML환자P-gp、LRP단독급이자공표체빈솔균교고.P-gp、LRP과도표체시노년AML환자적불량예후인소.
Objective To investigate the expression of P-glycoprotein(P-gp), multidrug resistance-associated protein (MRP), lung resistance-related protein (LRP) and predicts treatment outcome in the elderly acute myeloid leukemia. Methods Multi-parameter flow eytometric assay was used to quantify expression of P-go, muhidrug-associated resistance protein (MRP) and lung resistance-related protein (LRP) in bone marrow viable blasts from 12 eldely acute myeloid leukemia patients at diagnosis (M2a:4, one is come from MDS, M3a:2, M5a:5, M6a:1). Correlation of the MDR protein expression with treatment outcome were analysed. Results The frequency of expression of P-gp, MRP and LRP was 58.33 %, 8.33 %, 50 %, respectively and P-gp(+)/MRP(+) 0, P-gp(+)/LRP(+) 33.33 %, MRP(+)/LRP(+) 0, P-gp(+)/MRP(+)/LRP(+)8.33 %, P-go(-)/MRP (-)/LRP(-) 33.33 %;respectively. The frequency of overexpression of P-gp or LRP alone and both of them were relatively higher. The rate of complete remission (CR) and overall survival (OS) at one year of P-gp(+) group were significantly lower than those of P-go(-) group, and LRP(+) group also were lower than LRP(-) group. Conclusion The Frequency of expression of P-gp or LRP and coexpression of both of them were higher in elderly patients with AML. The overexpression of P-gp and/or LRP was a poor prognotic factor for eldely myeloid leukemia in elderly patients.