眼科研究
眼科研究
안과연구
CHINESE OPHTHALMIC RESEARCH
2010年
2期
167-170
,共4页
甲状腺相关眼病%前脂肪细胞%抵抗素
甲狀腺相關眼病%前脂肪細胞%牴抗素
갑상선상관안병%전지방세포%저항소
thyroid associated ophthalmopathy%preadiocyte%resistin
目的 探讨重组人抵抗素对甲状腺相关眼病(TAO)患者眼眶前脂肪细胞分化的影响及其在TAO发病中的可能作用.方法 10例严重TAO患者眼眶脂肪组织来自眼眶减压术中,正常对照组来自各种原因行眼球摘除术者.培养眼眶前脂肪细胞,并诱导分化,在分化液中分别加入0、10、25、50、100μg/L质量浓度的重组人抵抗素,采用逆转录PCR(RT PCR)法检测分化过程中过氧化物酶增生体激活受体γ(PPARγ)基因的表达,研究重组人抵抗素对前脂肪细胞分化的作用.结果 TAO患者及正常对照组眼眶前脂肪细胞在体外均可被诱导分化成脂肪细胞.分化成熟的脂肪细胞可被油红O染成橘红色.重组人抵抗素对眼眶前脂肪细胞的分化有抑制作用,RT PCR结果 显示PPARγ基因的表达随着抵抗素质量浓度的升高而减弱,差异有统计学意义(P＜0.05).结论 重组人抵抗素可抑制人眼眶脂肪组织来源前脂肪细胞的分化,脂肪细胞标记性PPARγ基因的表达减弱,有望在TAO治疗方面开创新的研究方向.
目的 探討重組人牴抗素對甲狀腺相關眼病(TAO)患者眼眶前脂肪細胞分化的影響及其在TAO髮病中的可能作用.方法 10例嚴重TAO患者眼眶脂肪組織來自眼眶減壓術中,正常對照組來自各種原因行眼毬摘除術者.培養眼眶前脂肪細胞,併誘導分化,在分化液中分彆加入0、10、25、50、100μg/L質量濃度的重組人牴抗素,採用逆轉錄PCR(RT PCR)法檢測分化過程中過氧化物酶增生體激活受體γ(PPARγ)基因的錶達,研究重組人牴抗素對前脂肪細胞分化的作用.結果 TAO患者及正常對照組眼眶前脂肪細胞在體外均可被誘導分化成脂肪細胞.分化成熟的脂肪細胞可被油紅O染成橘紅色.重組人牴抗素對眼眶前脂肪細胞的分化有抑製作用,RT PCR結果 顯示PPARγ基因的錶達隨著牴抗素質量濃度的升高而減弱,差異有統計學意義(P＜0.05).結論 重組人牴抗素可抑製人眼眶脂肪組織來源前脂肪細胞的分化,脂肪細胞標記性PPARγ基因的錶達減弱,有望在TAO治療方麵開創新的研究方嚮.
목적 탐토중조인저항소대갑상선상관안병(TAO)환자안광전지방세포분화적영향급기재TAO발병중적가능작용.방법 10례엄중TAO환자안광지방조직래자안광감압술중,정상대조조래자각충원인행안구적제술자.배양안광전지방세포,병유도분화,재분화액중분별가입0、10、25、50、100μg/L질량농도적중조인저항소,채용역전록PCR(RT PCR)법검측분화과정중과양화물매증생체격활수체γ(PPARγ)기인적표체,연구중조인저항소대전지방세포분화적작용.결과 TAO환자급정상대조조안광전지방세포재체외균가피유도분화성지방세포.분화성숙적지방세포가피유홍O염성귤홍색.중조인저항소대안광전지방세포적분화유억제작용,RT PCR결과 현시PPARγ기인적표체수착저항소질량농도적승고이감약,차이유통계학의의(P＜0.05).결론 중조인저항소가억제인안광지방조직래원전지방세포적분화,지방세포표기성PPARγ기인적표체감약,유망재TAO치료방면개창신적연구방향.
Background The enhance of differentiation of orbital preadipocyte is a main factor for pathogenesis of thyroid associated ophthalmopathy(TAO).Experiment has proved that orbital preadipocyte can differentiate into mature adipocyte expressing resistin,and resistin is a adipocyte factor with biological activity.Objective The present study is to investigate the effects of recombinant human resistin on differentiation of orbital preadipocytes derived from TAO patients orbital tissues.Methods Orbital adipose tissue was obtained from 10 eyes with TAO during the orbital decompression surgery.Orbital adipose tissue of the normal group was obtained from 5 removed eyes during the surgery due to different reasons.Preadipocytes were isolated and cultured using expand culture method.The 2-5 generations of preadipocytes were identified by oil red O staining under the light microscope.0,10,25,50,100ng/mL of recombinant human resistin were added into medium in the first day after differentiation of cells.The expression of PPARγ mRNA in differentiated adipocytes were detected by reverse trancripaton PCR(RT PCR).Results The differentiation of preadipocyte presented the similar procedure and outcome between the TAO group and normal group.Differentiated adipocyte was stained into the jacinth by oil red O.The expression of PPARγ mRNA in differentiated cells was gradually declined with the increase of concentration of recombinant human resistin (F=241.341,P＜0.01).A dose dependent inhibitory effect was detected in the expression of PPARγ mRNA.The weakest expression of PPARγ mRNA in differentiated cells was 100ng/mL resistin group.Conclusion The recombinant human resistin has an inhibitory effect on the differentiation of orbital preadipocytes by reducing the expression of adipose related genes.Resistin is probably a new research approach to treatment of TAO.
