CAJ | 학술논문

目的探讨滤泡辅助性T细胞(Tfh)在天疱疮和大疱性类天疱疮发病中的作用.方法天疱疮和大疱性类天疱疮患者各10例,与天疱疮和大疱性类天疱疮患者年龄、性别相匹配的正常人对照各 10例.采用流式细胞仪检测外周血单一核细胞(PBMC)中Tfh水平,采用酶联免疫吸附法(ELISA)检测其血清中白介素21(IL-21)水平,同时检测大疱性类天疱疮患者血清中抗BP180-NC16A抗体滴度,并进行抗体滴度与IL-21、Tfh水平的相关性分析.结果天疱疮患者组血清中IL-21水平(95.33±33.69 ng/L)显著高于其正常人对照组(54.50±18.13 ng/L)(t=3.38,P＜ 0.01),Tfh水平(12.08％±4.74％)亦显著高于其正常人对照组(6.15％±1.62％)(t=3.74,P＜ 0.01).大疱性类天疱疮患者组血清中IL-21水平(106.70±44.91 ng/L)显著高于其正常人对照组(55.37±15.89 ng/L)(t=3.41,P＜ 0.01),Tfh水平(11.85％±3.14％)亦显著高于其正常人对照组(6.03％±1.74％)(t=5.13,P＜ 0.01).大疱性类天疱疮组抗BP180-NC16A抗体滴度与IL-21水平呈正相关(r=0.77,P＜ 0.01),与Tfh水平亦呈正相关(r=0.67,P＜ 0.05).结论天疱疮和大疱性类天疱疮患者Tfh及其分泌的细胞因子IL-21水平均明显升高,可能在其发病过程中起一定的作用.
목적 탐토려포보조성T세포(Tfh)재천포창화대포성류천포창발병중적작용.방법 천포창화대포성류천포창환자각10례,여천포창화대포성류천포창환자년령、성별상필배적정상인대조각 10례.채용류식세포의검측외주혈단일핵세포(PBMC)중Tfh수평,채용매련면역흡부법(ELISA)검측기혈청중백개소21(IL-21)수평,동시검측대포성류천포창환자혈청중항BP180-NC16A항체적도,병진행항체적도여IL-21、Tfh수평적상관성분석.결과 천포창환자조혈청중IL-21수평(95.33±33.69 ng/L)현저고우기정상인대조조(54.50±18.13 ng/L)(t=3.38,P＜ 0.01),Tfh수평(12.08％±4.74％)역현저고우기정상인대조조(6.15％±1.62％)(t=3.74,P＜ 0.01).대포성류천포창환자조혈청중IL-21수평(106.70±44.91 ng/L)현저고우기정상인대조조(55.37±15.89 ng/L)(t=3.41,P＜ 0.01),Tfh수평(11.85％±3.14％)역현저고우기정상인대조조(6.03％±1.74％)(t=5.13,P＜ 0.01).대포성류천포창조항BP180-NC16A항체적도여IL-21수평정정상관(r=0.77,P＜ 0.01),여Tfh수평역정정상관(r=0.67,P＜ 0.05).결론 천포창화대포성류천포창환자Tfh급기분비적세포인자IL-21수평균명현승고,가능재기발병과정중기일정적작용.
Objective To explore the roles of follicular helper T cells (Tfh) in the pathogenesis of pemphigus and bullous pemphigoid (BP).Methods Venous blood was collected from 20 patients (including 10 cases of pemphigus and 10 cases of BP) and 20 age- and sex-matched normal human controls.Enzyme linked immunosorbent assay (ELISA) was performed to measure the levels of serum interleukin (IL)-21 and anti-BP180-NC16A antibody,and flow cytometry to detect the percentage of Tfh in peripheral blood mononuclear cells (PBMCs).The relationship of anti-BP180-NC16A antibody titer to Tfh and IL-21 levels was assessed by linear correlation analysis.Results The serum IL-21 level and Tfh percentage in PBMCs were significantly higher in patients with pemphigus than in the normal controls (95.33 ± 33.69 vs.54.50 ± 18.13 pg/ml,t =3.38,P＜ 0.01;12.08％ ± 4.74％ vs.6.15％ ± 1.62％,t =3.74,P ＜ 0.01),and higher in patients with BP than in the normal controls ( 106.70 ± 44.91 vs.55.37 ± 15.89 pg/ml,t =3.41,P ＜ 0.01; 11.85％ ± 3.14％ vs.6.03％ ± 1.74％,t =5.13,P ＜ 0.01 ).The titer of anti-BP180-NC 16A antibody was positively correlated with the percentage of Tfh (r =0.67,P＜ 0.05) and serum level of IL-21 (r=0.77,P＜ 0.01) in patients with BP.Conclusions There is a significant increase in the percentage of Tfh and serum level of IL-21 in patients with pemphigus and BP,hinting a certain role of Tfh and IL-21 in the pathogenesis of pemphigus and BP.