中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
1期
51-53
,共3页
脓毒症%基因多态性
膿毒癥%基因多態性
농독증%기인다태성
Sepsis%Genetic polymorphisms
目的 探讨脓毒症易感性与IRAK-M基因多态性的关系.方法 选择脓毒症患者82例为实验组,118例健康人群为对照组.应用聚合酶链反应(PCR)-限制性片段长度多态性分析法(RFLP)分析IRAK-M+22148G＞A基因多态性.结果 脓毒症组IRAK-M+22148G＞A位点G/G基因型频率高于对照组(81.7%比28.8%),差异有统计学意义(P＜0.01),G等位基因频率高于对照组(84.1%比33.9%,P＜0.01),差异有统计学意义,脓毒症组肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6水平高于对照组[(843.00±97.34)ng/L比(287.00±79.12)ng/L;(741.00±65.61)ng/L比(194.00±58.47)ng/L],差异有统计学意义(P＜0.05);G/G基因型与脓毒症之间有明显相关(OR=11.03,95%CI:5.55～21.94).结论 IRAK-M+22148G/A基因多态性与脓毒症的易感性相关,G/G基因型者易患脓毒症.
目的 探討膿毒癥易感性與IRAK-M基因多態性的關繫.方法 選擇膿毒癥患者82例為實驗組,118例健康人群為對照組.應用聚閤酶鏈反應(PCR)-限製性片段長度多態性分析法(RFLP)分析IRAK-M+22148G＞A基因多態性.結果 膿毒癥組IRAK-M+22148G＞A位點G/G基因型頻率高于對照組(81.7%比28.8%),差異有統計學意義(P＜0.01),G等位基因頻率高于對照組(84.1%比33.9%,P＜0.01),差異有統計學意義,膿毒癥組腫瘤壞死因子(TNF)-α、白細胞介素(IL)-6水平高于對照組[(843.00±97.34)ng/L比(287.00±79.12)ng/L;(741.00±65.61)ng/L比(194.00±58.47)ng/L],差異有統計學意義(P＜0.05);G/G基因型與膿毒癥之間有明顯相關(OR=11.03,95%CI:5.55～21.94).結論 IRAK-M+22148G/A基因多態性與膿毒癥的易感性相關,G/G基因型者易患膿毒癥.
목적 탐토농독증역감성여IRAK-M기인다태성적관계.방법 선택농독증환자82례위실험조,118례건강인군위대조조.응용취합매련반응(PCR)-한제성편단장도다태성분석법(RFLP)분석IRAK-M+22148G＞A기인다태성.결과 농독증조IRAK-M+22148G＞A위점G/G기인형빈솔고우대조조(81.7%비28.8%),차이유통계학의의(P＜0.01),G등위기인빈솔고우대조조(84.1%비33.9%,P＜0.01),차이유통계학의의,농독증조종류배사인자(TNF)-α、백세포개소(IL)-6수평고우대조조[(843.00±97.34)ng/L비(287.00±79.12)ng/L;(741.00±65.61)ng/L비(194.00±58.47)ng/L],차이유통계학의의(P＜0.05);G/G기인형여농독증지간유명현상관(OR=11.03,95%CI:5.55～21.94).결론 IRAK-M+22148G/A기인다태성여농독증적역감성상관,G/G기인형자역환농독증.
Objective To determine the association between the genetic polymorphisms of IRAK-M and the susceptivity of sepsis. Methods Two candidate gene loci in + 22148G ＞ A patients with 82 sepsis infection and 118 heahhy controls were investigated. The polymorphisms were assessed by the polymerase chain reaction (PCR) and the restrict fragment length polymorphisms (RFLP). Results In sepsis group and control group, the frequency of G/G gene was 81.7% and 8. 8% ( P ＜0. 01 ) and that of G allele was 84.1% and 33.9% ( P ＜0. 01 ), respectively. The levels of tumor necrosis factor (TNF) -α and interleukin (IL) -6 in sepsis group were higher than in control group [ ( 843.00±97.34) vs (287.00±79.12) ng/L;(741.00±65.61) vs (194.00±58.47)ng/L,P＜0.05].The G/G genotype was associated with sepsis (OR=11.03,95% CI=5.55-21.94).Conclusion The genetic polymorphism of +22148 site of IRAK-M gene is associated with the susceptivity of sepsis.The G/G genotype is susceptive to sepsis.
