CAJ | 학술논문

背景:周围神经损伤能致脊髓神经元的凋亡,严重影响神经修复后功能的恢复.电刺激能有效促进神经的再生,但对神经元凋亡的影响研究较少.目的:观察直流电场对鼠坐骨神经切断后脊髓运动神经元bcl-2,bcl-X/L,bax及c-jun表达的作用,探讨电刺激对预防细胞凋亡的机制.设计:采用随机对照实验研究.地点和对象:实验在上海同济大学附属铁路医院骨科完成,对象为雄性SD大鼠30只,6周龄,体质量180～220 g.干预:将雄性SD大鼠随机分为对照组和实验组,每组15只大鼠.用10g/L硫喷妥钠(5 mg/100g)腹腔麻醉后,在大鼠右侧臀肌间隙显露坐骨神经,距梨状肌下缘0.5 cm处将坐骨神经切断,近端以5-0尼龙线作双重结扎,远端切除1 cm后埋入股二头肌中,关闭切口.沿棘突后方正中切口,在T1o～L3右侧椎板及棘突间钝性剥离骶棘肌,切除椎板,将电刺激器阳性盘状电极板置入T10椎板下硬膜囊之腹侧,阴性盘状电极板置入L3椎板上硬膜囊背侧,对照组置入无输出电流的刺激器,分批于术后1,4,7,14,28 d取材.实验操作由同一组人员完成.主要观察指标:脊髓bcl-2,bcl-X/L,bax及c-jun的阳性运动神经元数.结果:坐骨神经切断后4,7,14 d,电刺激组脊髓运动神经元bcl-2阳性数分别为(28.67±1.53),(34.67±1.53),(41.33±1.53)个;bcl-X/L阳性数分别为(23.00±4.36),(32.67±2.52),(44.33±2.08)个,均高于对照组相应阳性数(19.67±1.53),(25.67±2.08),(33.67±1.53);(15.33±0.58),(36.33±2.52),(33.00±4.36).坐骨神经切断后4,7,14,28d,电刺激脊髓运动神经元bax阳性数均低于对照组,坐骨神经切断后7,14,28 d,电刺激脊髓运动神经元c-jun阳性数均少于对照组.结论:直流电刺激对鼠坐骨神经切断后脊髓运动神经元bcl-2,bcl-X/L,bax及c-jun的表达具有调节作用.
배경:주위신경손상능치척수신경원적조망,엄중영향신경수복후공능적회복.전자격능유효촉진신경적재생,단대신경원조망적영향연구교소.목적:관찰직류전장대서좌골신경절단후척수운동신경원bcl-2,bcl-X/L,bax급c-jun표체적작용,탐토전자격대예방세포조망적궤제.설계:채용수궤대조실험연구.지점화대상:실험재상해동제대학부속철로의원골과완성,대상위웅성SD대서30지,6주령,체질량180～220 g.간예:장웅성SD대서수궤분위대조조화실험조,매조15지대서.용10g/L류분타납(5 mg/100g)복강마취후,재대서우측둔기간극현로좌골신경,거리상기하연0.5 cm처장좌골신경절단,근단이5-0니룡선작쌍중결찰,원단절제1 cm후매입고이두기중,관폐절구.연극돌후방정중절구,재T1o～L3우측추판급극돌간둔성박리저극기,절제추판,장전자격기양성반상전겁판치입T10추판하경막낭지복측,음성반상전겁판치입L3추판상경막낭배측,대조조치입무수출전류적자격기,분비우술후1,4,7,14,28 d취재.실험조작유동일조인원완성.주요관찰지표:척수bcl-2,bcl-X/L,bax급c-jun적양성운동신경원수.결과:좌골신경절단후4,7,14 d,전자격조척수운동신경원bcl-2양성수분별위(28.67±1.53),(34.67±1.53),(41.33±1.53)개;bcl-X/L양성수분별위(23.00±4.36),(32.67±2.52),(44.33±2.08)개,균고우대조조상응양성수(19.67±1.53),(25.67±2.08),(33.67±1.53);(15.33±0.58),(36.33±2.52),(33.00±4.36).좌골신경절단후4,7,14,28d,전자격척수운동신경원bax양성수균저우대조조,좌골신경절단후7,14,28 d,전자격척수운동신경원c-jun양성수균소우대조조.결론:직류전자격대서좌골신경절단후척수운동신경원bcl-2,bcl-X/L,bax급c-jun적표체구유조절작용.
BACKGROUND: The injury of peripheral nerve would induce apoptosis ofspinal neuron, which affect severely recovery of nerve function after rehabilitation. It is proved that current stimulation can promote regeneration ofnerve, but it is scarcely reported on the effect of current stimulation onneuron apoptosis.OBJECTIVE: To observe the effect of direct-current field stimulation onbcl-2, bcl-X/L, bax and c-jun gene expression in spinal cord neurons aftertransection of sciatic nerve and to discuss the mechanism of preventive effectof electric stimulation on apoptosis.DESIGN: A randomized controlled experiment conducted.SETTING and PARTICIPANTS: The experiment was performed in theDepartment of Orthopaedics, the Affiliated Railway Hospital, Tongji University. A total of 30 male SD rats(6-week old, body mass of 180-220 g) wereexperimental objects.INTERVENTIONS: Thirty male SD rats were divided into experimentalgroup and control group randomly, 15 rats in each group. After abdominalanaesthetizing with penthiobarbital of 10 g/L concentration, the sciaticnerve was exposed at the interspace of right gluteus. It was transected 0. 5cm inferior to piriformis with proximal end ligtured by two nylon thread of5-0 and distal end cut off by 1 cm length and embedded into biceps formoris. Then the incision was closed. Another median incision was made along spinal process. The muscle on right lamina and spinal process of T10-L3 was blunt dissected. After laminectomy, the discal anode of stimulatorwas put into the abdominal side of the dura under lamina of T10, the cathodedorsal side of dura under lamina of L3. In the control group, stimulatorswithout deferent current were put in the same way. The specimens weretaken 1, 4, 7, 14 and 28 days after operation. Experiments were performed bythe same group of people.MAIN OUTCOME MEASURES: With immunohistochemistry method(ABC method), the gene expression of bcl-2, bcl-X/L, bax and c-jun wouldbe investigated and motoneurons of spinal cord were numbered under lightmicroscope.RESULTS: 4, 7, 14 days after operation in theexperimental group, thenumber of positive spinal cord motor neurons with bcl-2, and bcl-X/L geneexpression were 28.67 ± 1.53, 34.67 ± 1.53, 41.33 ± 1.53 and 23.00 ±4. 36, 32.67 ±2.52, 44. 33 ±2.08, higher than controlled group(19.67 ±1.53,25.67 ± 2.08,33.67 ± 1.53; 15.33 ±0. 58,36. 33 ±2. 52,33.00 ±4.36). 4, 7, 14 and 28 days after operation, the number of positive spinalcord motor neurons with bax were lower than controlled group. 4, 7, 28 daysafter operation, the number of positive spinal cord motor neurons with c-junwere lower than controlled group.CONCLUSION: Direct-current field may modulate the expression of bcl-2,bcl-X/L, bax and c-jun gene after sciatic nerves transection in rats.