CAJ | 학술논문

应用免疫组织化学和Western blot技术对雌激素受体β(Estrogen Receptor,β,ERβ)及其磷酸化形式p-ERβ进行定位和半定量的研究,探讨ERβ和p-ERβ在小鼠发情周期卵巢中的表达规律.Western bolt检测结果表明,在小鼠发情周期卵巢中,ERβ和p-ERβ均在小鼠发情期卵巢中高水平表达,而在其他时期的表达量差异不显著.同时.免疫组织化学结果显示,ERβ及p-ERβ在小鼠卵巢的卵泡和黄体中,随发情周期的变化而有规律的分布.ERβ及p-ERβ在小鼠卵巢中的分布和表达量的变化与卵巢中卵泡和黄体的发育过程基本一致,揭示ERβ对小鼠卵巢发情周期的周期变化具有调节作用.
응용면역조직화학화Western blot기술대자격소수체β(Estrogen Receptor,β,ERβ)급기린산화형식p-ERβ진행정위화반정량적연구,탐토ERβ화p-ERβ재소서발정주기란소중적표체규률.Western bolt검측결과표명,재소서발정주기란소중,ERβ화p-ERβ균재소서발정기란소중고수평표체,이재기타시기적표체량차이불현저.동시.면역조직화학결과현시,ERβ급p-ERβ재소서란소적란포화황체중,수발정주기적변화이유규률적분포.ERβ급p-ERβ재소서란소중적분포화표체량적변화여란소중란포화황체적발육과정기본일치,게시ERβ대소서란소발정주기적주기변화구유조절작용.
In the experiment, the expression and distribution regulation of Erβ and p-Erβ were researched on mouse ovary which were chosen from different pedods of cestrous cycle. Dudng the study process, immuno-histochemistry and Western blot technique were used as the main investigative methods, which were respective to study on protein location and expressive amount of Erβ and p,-Erβ in mouse ovary, results showed that, in different mouse cestrous cycle, Erβ and p-Erβ are highly express in whole estrus. The than other three pedods by Western blot technique, while there was no distiction among other pededs. And immuno-histochemistry results revealed that in ovarian follicle and corepus luteum of mouse ovary, Erβ and p-Erβ were distributed regularly during mouse oestrous cycle. The experiment confirmed that in the pedod of mouse ovary, distribution and expressive of Erβ and p-Erβ amount change were coincident with development process of ovarian follicle and corepus luteum. And the experiment revealed that Erβ played an important role in regulating the reproductive process of mouse ovary.