哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2009年
2期
130-133,136
,共5页
E-选择素%鼻黏膜耐受%脑缺血-再灌注损伤
E-選擇素%鼻黏膜耐受%腦缺血-再灌註損傷
E-선택소%비점막내수%뇌결혈-재관주손상
E-selectin%nasal mucosal toleration%cerebral ischemia-reperfusion injury
目的 探讨E-选择素鼻黏膜耐受对大鼠局灶脑缺血再灌注损伤的影响及其发生的可能机制.方法 采用3种处理方法,在大鼠鼻内滴人磷酸盐缓冲溶液(PBS)、E-选择素或不滴入任何药物.诱导方案结束后,建立大鼠脑缺血2 h再灌注22 h模型,用流式细胞仪检测血中CD3~+CD4~+T、CD3~+CD8~+T细胞的表达,计算机检测脑梗死体积,用逆转录-聚合酶链反应(RT-PCR)检测脑缺血再灌注组织中白细胞介素-10(IL-10)及转化生长因子-B1(TGF-β1)mRNA表达.结果 加强诱导组中E-选择素组较其他2组比较,以及E-选择素的加强诱导组与单程诱导组比较均表现为CD3~+CD4~+T淋巴细胞表达无差别(P>0.05),CD3~+CD8~+T表达明显减少(P<0.05),脑梗死体积明显缩小(P<0.05),IL-10 mRNA的表达增加(P<0.05),TGF-131 mRNA表达呈增加趋势(0.05<P<0.1).单程诱导组中各组比较不具有统计学意义.结论 通过E-选择素鼻黏膜加强诱导耐受的方法能够诱导免疫耐受的发生,释放抗炎细胞因子,明显减轻大鼠脑缺血再灌注后的损伤.
目的 探討E-選擇素鼻黏膜耐受對大鼠跼竈腦缺血再灌註損傷的影響及其髮生的可能機製.方法 採用3種處理方法,在大鼠鼻內滴人燐痠鹽緩遲溶液(PBS)、E-選擇素或不滴入任何藥物.誘導方案結束後,建立大鼠腦缺血2 h再灌註22 h模型,用流式細胞儀檢測血中CD3~+CD4~+T、CD3~+CD8~+T細胞的錶達,計算機檢測腦梗死體積,用逆轉錄-聚閤酶鏈反應(RT-PCR)檢測腦缺血再灌註組織中白細胞介素-10(IL-10)及轉化生長因子-B1(TGF-β1)mRNA錶達.結果 加彊誘導組中E-選擇素組較其他2組比較,以及E-選擇素的加彊誘導組與單程誘導組比較均錶現為CD3~+CD4~+T淋巴細胞錶達無差彆(P>0.05),CD3~+CD8~+T錶達明顯減少(P<0.05),腦梗死體積明顯縮小(P<0.05),IL-10 mRNA的錶達增加(P<0.05),TGF-131 mRNA錶達呈增加趨勢(0.05<P<0.1).單程誘導組中各組比較不具有統計學意義.結論 通過E-選擇素鼻黏膜加彊誘導耐受的方法能夠誘導免疫耐受的髮生,釋放抗炎細胞因子,明顯減輕大鼠腦缺血再灌註後的損傷.
목적 탐토E-선택소비점막내수대대서국조뇌결혈재관주손상적영향급기발생적가능궤제.방법 채용3충처리방법,재대서비내적인린산염완충용액(PBS)、E-선택소혹불적입임하약물.유도방안결속후,건립대서뇌결혈2 h재관주22 h모형,용류식세포의검측혈중CD3~+CD4~+T、CD3~+CD8~+T세포적표체,계산궤검측뇌경사체적,용역전록-취합매련반응(RT-PCR)검측뇌결혈재관주조직중백세포개소-10(IL-10)급전화생장인자-B1(TGF-β1)mRNA표체.결과 가강유도조중E-선택소조교기타2조비교,이급E-선택소적가강유도조여단정유도조비교균표현위CD3~+CD4~+T림파세포표체무차별(P>0.05),CD3~+CD8~+T표체명현감소(P<0.05),뇌경사체적명현축소(P<0.05),IL-10 mRNA적표체증가(P<0.05),TGF-131 mRNA표체정증가추세(0.05<P<0.1).단정유도조중각조비교불구유통계학의의.결론 통과E-선택소비점막가강유도내수적방법능구유도면역내수적발생,석방항염세포인자,명현감경대서뇌결혈재관주후적손상.
0bjective To study the effect of mucosal toleration inducted bv E-selectin on cerebral ischemia reperfusion injury in rats and its mechanism.Methods According to the single and booster toleration schedules,phosphate buffered solution(PBS)or E-selectin instilled into each nostril of rats,or rats no drug was administrated.After the toleration schedules were fininshed,Zea-longa operation was referred to make the model of focal cerebral ischemia-reperfusion.The expressions of CD3~+CD4~+T and CD3~+CD8~+T lymphocyte subgroup were measured with flow cytometry.The volumes of cerebral infarction were measured by computer tomography.The expressions of IL-10 and TGF-β1 mRNA in cerebral ischemia-reperfusion region were evaluated with RT-PCR.Results The numbers of CD3~+CD4~+T lymphocyte had no significant difference(P>0.05)and CD3~+CD8~+T cells were obviously suppressed (P<0.05),as compared the E-selectin group with the other two groups in booster toleration group,the infarction volumes were effectively decreased(P<0.05).the expression of IL-10 mRNA was significantly raised up(P<0.05),the expression of TGF-131 mRNA had a slight trend toward an increase(P>0.05).There was the same result between E-selectin booster-toleration group and singletoleration group.But the levels of CD3~+CD4~+T cells、CD3~+CD8~+T cells、the infarction volumes、IL10 and TGF-β1 mRNA had no significant differences among single-toleration groups.Conclusion MUCOSal booster toleration schedules to E-selectin can induce immunologic tolerance and release antiinflammatory cytokines.the brain damage is significantly decreased after reginoal cerebral ischemia reperfusion in rats.
