中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2008年
9期
956-960
,共5页
郑悦亮%夏志敏%周辉%赵万军
鄭悅亮%夏誌敏%週輝%趙萬軍
정열량%하지민%주휘%조만군
椎板切除术%瘢痕%转化生长因子-β3%Smad 6
椎闆切除術%瘢痕%轉化生長因子-β3%Smad 6
추판절제술%반흔%전화생장인자-β3%Smad 6
Laminectoray%Cicatrix%Transforming growth factor-β%Smad
目的 探讨透明质酸钠对硬膜外瘢痕中转化生长因子(transforming growth Factor,TGF)-β3和Smad6表达水平的影响.方法 雄性SD大鼠60只随机分成空白对照组(A)、透明质酸钠组(B),每组30只.手术切除第4、5腰椎椎板,造成0.25 cm×1.00 cm硬脊膜裸露区,B组于硬膜囊外均匀地涂上一层透明质酸钠约0.3 ml,A组以等量生理盐水处理.术后第2、4、8周末处死动物,逆转录酶链反应(Reverse Transcription Polymerase Chain Reaction,RT-PCR)测定其硬膜外瘢痕组织中TGF-β3和Smad6的表达水平,按Rydell法评定粘连程度,透射电镜观察其超微结构.大体观察数据的比较用两个独立样本比较的秩和检验(Mann-Whitney U检验);TGF-β3和Smad6基因表达量的数据(x±s)用完全随机设计两样本均数的t检验.结果 第2周时,两组TGF-β3 mRNA表达均较低,均数分别为0.22和0.257(P=0.027);第4周时,B组TGV-β3的表达明显增高,两组均数分别为0.362和0.411(P=0.006);第8周时,A组TGF-β3 mRNA的表达水平虽也有明显增高,但两组间仍存在明显的差异,分别为0.427和0.470(P=0.015).Smad 6 mRNA的表达趋势与TGF-β3类似,第2周、第4周、第8周分别为0.169和0.205(P=0.089)、0.294和0.351(P=0.031)、0.469和0.543(P=0.021).大体观察也发现B组粘连程度明显降低(P＜0.05).透射电镜示各时相B组瘢痕中成纤维细胞以及细胞内的细胞器的数量均减少,胶原纤维的合成受到抑制.结论 透明质酸钠通过上调TCF-β3和Smad 6的表达,减少了成纤维细胞增殖和胶原等细胞外基质(Extracellular Matrix,ECM)的沉积,达到了预防硬膜外瘢痕增生、粘连的效果.
目的 探討透明質痠鈉對硬膜外瘢痕中轉化生長因子(transforming growth Factor,TGF)-β3和Smad6錶達水平的影響.方法 雄性SD大鼠60隻隨機分成空白對照組(A)、透明質痠鈉組(B),每組30隻.手術切除第4、5腰椎椎闆,造成0.25 cm×1.00 cm硬脊膜裸露區,B組于硬膜囊外均勻地塗上一層透明質痠鈉約0.3 ml,A組以等量生理鹽水處理.術後第2、4、8週末處死動物,逆轉錄酶鏈反應(Reverse Transcription Polymerase Chain Reaction,RT-PCR)測定其硬膜外瘢痕組織中TGF-β3和Smad6的錶達水平,按Rydell法評定粘連程度,透射電鏡觀察其超微結構.大體觀察數據的比較用兩箇獨立樣本比較的秩和檢驗(Mann-Whitney U檢驗);TGF-β3和Smad6基因錶達量的數據(x±s)用完全隨機設計兩樣本均數的t檢驗.結果 第2週時,兩組TGF-β3 mRNA錶達均較低,均數分彆為0.22和0.257(P=0.027);第4週時,B組TGV-β3的錶達明顯增高,兩組均數分彆為0.362和0.411(P=0.006);第8週時,A組TGF-β3 mRNA的錶達水平雖也有明顯增高,但兩組間仍存在明顯的差異,分彆為0.427和0.470(P=0.015).Smad 6 mRNA的錶達趨勢與TGF-β3類似,第2週、第4週、第8週分彆為0.169和0.205(P=0.089)、0.294和0.351(P=0.031)、0.469和0.543(P=0.021).大體觀察也髮現B組粘連程度明顯降低(P＜0.05).透射電鏡示各時相B組瘢痕中成纖維細胞以及細胞內的細胞器的數量均減少,膠原纖維的閤成受到抑製.結論 透明質痠鈉通過上調TCF-β3和Smad 6的錶達,減少瞭成纖維細胞增殖和膠原等細胞外基質(Extracellular Matrix,ECM)的沉積,達到瞭預防硬膜外瘢痕增生、粘連的效果.
목적 탐토투명질산납대경막외반흔중전화생장인자(transforming growth Factor,TGF)-β3화Smad6표체수평적영향.방법 웅성SD대서60지수궤분성공백대조조(A)、투명질산납조(B),매조30지.수술절제제4、5요추추판,조성0.25 cm×1.00 cm경척막라로구,B조우경막낭외균균지도상일층투명질산납약0.3 ml,A조이등량생리염수처리.술후제2、4、8주말처사동물,역전록매련반응(Reverse Transcription Polymerase Chain Reaction,RT-PCR)측정기경막외반흔조직중TGF-β3화Smad6적표체수평,안Rydell법평정점련정도,투사전경관찰기초미결구.대체관찰수거적비교용량개독립양본비교적질화검험(Mann-Whitney U검험);TGF-β3화Smad6기인표체량적수거(x±s)용완전수궤설계량양본균수적t검험.결과 제2주시,량조TGF-β3 mRNA표체균교저,균수분별위0.22화0.257(P=0.027);제4주시,B조TGV-β3적표체명현증고,량조균수분별위0.362화0.411(P=0.006);제8주시,A조TGF-β3 mRNA적표체수평수야유명현증고,단량조간잉존재명현적차이,분별위0.427화0.470(P=0.015).Smad 6 mRNA적표체추세여TGF-β3유사,제2주、제4주、제8주분별위0.169화0.205(P=0.089)、0.294화0.351(P=0.031)、0.469화0.543(P=0.021).대체관찰야발현B조점련정도명현강저(P＜0.05).투사전경시각시상B조반흔중성섬유세포이급세포내적세포기적수량균감소,효원섬유적합성수도억제.결론 투명질산납통과상조TCF-β3화Smad 6적표체,감소료성섬유세포증식화효원등세포외기질(Extracellular Matrix,ECM)적침적,체도료예방경막외반흔증생、점련적효과.
Objective To study the effect of sodium hyaluronate on the expression of transforming growth factor-β3(TGF-β3) and Smad 6 of the epidural scar tissue. Method Totally 60 male SD rats were randomly divided into two groups:the control group(A, n = 30), sodium hyaluronate group(B, n = 30). 0.25 × 1 cm2 dura mater uncovered area laminectomy was performed at L4 and L5, covered with 0.3 ml sodium hyaluronate in group B, covered with same amount of saline in group A. The animals were sacrificed at 2, 4 and 8 weeks after operation. The specimens were prepared for determination of the expression of TGF-β3 and Smad 6 at scar tissue and the degree of scar adhesion according to Rydell method, and observed the ultrastructure changes of scar tissue with transmission electron microscope. Results At 2 weeks after operation, the expression of TGF-β3 mRNA of two groups were 0.22 and 0.257 ( P = 0.027), respectively. At 4 weeks, group B was was increased significantly, and the mean numbers were 0.362 and 0.411 (P = 0.006). At 8 weeks, the expression of TGF-β3 mRNA of group A was increased significantly, too, but the difference between two groups was significantly, they were 0.427 and 0.470 (P =0.015), respectively. The trend of the expression of TGF-β3 and Smad 6 mRNA was similar. At 2, 4, 8, weeks, the expression was 0.169 and 0.205 (P = 0.089), 0.294 and 0.351 (P = 0.031), and 0.469 and 0.543 (P = 0.021), respectively.In group B the duramater adhesion was decreased (P ＜ 0.05), the proliferation of fibroblasts and fihroblastic function were inhibited (P ＜ 0.05). Conclusions Sodium hyaluronate up-regulated the expression of TGF-β3 and Smad6 and reduced the proliferation and collagen synthesis in fibroblast culture in the scar tissue.
