CAJ | 학술논문

目的探讨糖尿病足溃疡大鼠血锌水平及金属硫蛋白( metallothionein,MT)的表达对清除自由基及抗氧化作用的意义.方法将60只雄性Wistar大鼠随机分为对照组(20只)和造模组(40只),其中造模组链脲佐菌素尾静脉注射诱导糖尿病,对照组注射等量柠檬酸-柠檬酸钠缓冲液.将两组大鼠足背部一矩形全层皮肤组织切除,建立糖尿病足溃疡大鼠模型,监测两组大鼠血锌水平变化,应用免疫组化技术检测MT的表达.结果造模组大鼠血锌水平(1.17 ±0.09)较对照组( 1.48±0.07)明显减低,差异有统计学意义(t=12.94,P＜0.01).免疫组化研究发现在对照组大鼠有少量MT表达,而造模组大鼠MT的表达明显增高,差异有统计学意义(x2=12.78,P＜0.01).结论糖尿病足溃疡大鼠的血锌水平及MT的表达情况可能与清除自由基、抗氧化作用有关,MT的表达增高对防止糖尿病足溃疡病变进一步发展可能具有重要作用.
목적 탐토당뇨병족궤양대서혈자수평급금속류단백( metallothionein,MT)적표체대청제자유기급항양화작용적의의.방법 장60지웅성Wistar대서수궤분위대조조(20지)화조모조(40지),기중조모조련뇨좌균소미정맥주사유도당뇨병,대조조주사등량저몽산-저몽산납완충액.장량조대서족배부일구형전층피부조직절제,건립당뇨병족궤양대서모형,감측량조대서혈자수평변화,응용면역조화기술검측MT적표체.결과 조모조대서혈자수평(1.17 ±0.09)교대조조( 1.48±0.07)명현감저,차이유통계학의의(t=12.94,P＜0.01).면역조화연구발현재대조조대서유소량MT표체,이조모조대서MT적표체명현증고,차이유통계학의의(x2=12.78,P＜0.01).결론 당뇨병족궤양대서적혈자수평급MT적표체정황가능여청제자유기、항양화작용유관,MT적표체증고대방지당뇨병족궤양병변진일보발전가능구유중요작용.
diabetic foot ulcer of rats induced by streptozotosin,and to find the significance of free radical scavenging and the antioxidative function.Methods A total of 60 male wistar rats were randomized into the control group (20 rats) and diabetic model group (40 rats).The model group was induced to diabetic foot ulcer using streptozotosin tail intravenous injection,and the control group was injected equal citric acid-citric acid sodium buffer fluid instead.A rectangular full-thickness skin of dorsum of foot in the two groups was removed from model rats to construct diabetic foot ulcer.Serum zinc levels of the two groups were monitored,and immunohistochemical technique was used to detect the MT expression.Results Serum zinc levels in the model group( 1.17 ±0.09) was significantly lower than control group( 1.48 ±0.07),and the difference was statistically significant ( t =12.94,P ＜0.01 ).The MT expression in control group was low,while it significantly increased in diabetic model group,and the difference was statistically significant ( x2 =12.78,P ＜ 0.01 ).Conclusions The zinc levels and MT expression of diabetic foot ulcer rats may be associated with free radical scavenging and the antioxidative function.The increased expression of MT may play an important role in preventing the further development of diabetic foot ulcer disease.