中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2008年
5期
436-438
,共3页
徐卫国%宋纪宁%杨小青%郝世柱%胡潺潺%李明%吴春涛%朱红成%李瑞伟%赵光明
徐衛國%宋紀寧%楊小青%郝世柱%鬍潺潺%李明%吳春濤%硃紅成%李瑞偉%趙光明
서위국%송기저%양소청%학세주%호잔잔%리명%오춘도%주홍성%리서위%조광명
大肠癌%COX-2%CD105%肿瘤血管密度%新生血管形成
大腸癌%COX-2%CD105%腫瘤血管密度%新生血管形成
대장암%COX-2%CD105%종류혈관밀도%신생혈관형성
Colorectal carcinoma%COX-2%CD105%MVD%Angiogenesis
目的 探讨COX-2的表达与肿瘤血管密度(MVD)值及临床病理特征的关系,以揭示COX-2在肿瘤的形成、发展和新生血管形成过程中所发挥的作用.方法 采用免疫组织化学法和RT-PCR法测定45例标本的COX-2表达水平,采用CD105标记肿瘤组织中的新生血管计算MVD值,分析COX-2、MVD值与大肠癌生物学性质的关系,分析COX-2表达与MVD的相关性.结果COX-2 mRNA和蛋白的表达在肿瘤是否有淋巴结转移,Dukes分期和肿瘤的不同分化程度各个分组之间差异有统计学意义(均P＜0.05).MVD值在肿瘤是否有淋巴结转移、Dukes分期、浸润深度各个分组之间差异均有统计学意义(均P＜0.05).大肠癌中COX-2在蛋白水平和基因转录水平的表达均与MVD值有正相关(P＜0.05).结论COX-2可能在大肠癌的发生和发展中起重要作用,COX-2促进新生血管的生成,从而促进大肠癌的浸润和转移.
目的 探討COX-2的錶達與腫瘤血管密度(MVD)值及臨床病理特徵的關繫,以揭示COX-2在腫瘤的形成、髮展和新生血管形成過程中所髮揮的作用.方法 採用免疫組織化學法和RT-PCR法測定45例標本的COX-2錶達水平,採用CD105標記腫瘤組織中的新生血管計算MVD值,分析COX-2、MVD值與大腸癌生物學性質的關繫,分析COX-2錶達與MVD的相關性.結果COX-2 mRNA和蛋白的錶達在腫瘤是否有淋巴結轉移,Dukes分期和腫瘤的不同分化程度各箇分組之間差異有統計學意義(均P＜0.05).MVD值在腫瘤是否有淋巴結轉移、Dukes分期、浸潤深度各箇分組之間差異均有統計學意義(均P＜0.05).大腸癌中COX-2在蛋白水平和基因轉錄水平的錶達均與MVD值有正相關(P＜0.05).結論COX-2可能在大腸癌的髮生和髮展中起重要作用,COX-2促進新生血管的生成,從而促進大腸癌的浸潤和轉移.
목적 탐토COX-2적표체여종류혈관밀도(MVD)치급림상병리특정적관계,이게시COX-2재종류적형성、발전화신생혈관형성과정중소발휘적작용.방법 채용면역조직화학법화RT-PCR법측정45례표본적COX-2표체수평,채용CD105표기종류조직중적신생혈관계산MVD치,분석COX-2、MVD치여대장암생물학성질적관계,분석COX-2표체여MVD적상관성.결과COX-2 mRNA화단백적표체재종류시부유림파결전이,Dukes분기화종류적불동분화정도각개분조지간차이유통계학의의(균P＜0.05).MVD치재종류시부유림파결전이、Dukes분기、침윤심도각개분조지간차이균유통계학의의(균P＜0.05).대장암중COX-2재단백수평화기인전록수평적표체균여MVD치유정상관(P＜0.05).결론COX-2가능재대장암적발생화발전중기중요작용,COX-2촉진신생혈관적생성,종이촉진대장암적침윤화전이.
Objective To investigate whether cyclooxygenase-2(COX-2)expression are related to the clinicopathological features,and to microvessel density(MVD).Methods COX-2 expressions were detected in 45colorectal carcinoma samples by immunostaining.MVD was evaluated with the specific antibody CD105.Quantitationof COX-2 mRNA was performed by RT-PCR in 45 specimens of colorectal carcinoma.Results The expressions of COX-2 mRNA and protein were significant variation among lymphatic metastasis,high Dukes stages and grade of differation(P＜0.05);Increased value of MVD were significant variation among lymphatic metastasis,high Dukes stage and depth of tumor invasion(P＜0.05).Both the overexpression of COX-2 protein and mRNA was positively correlated with the value of MVD(P＜0.05).Conclusion COX-2 plays an important role in the development of colorectal carcinoma.COX-2 may up-regulate the value of MVD,which induces angiogenesis and accordingly contributes to metastasis.
