中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2010年
3期
152-154,160
,共4页
张成雷%孙清慧%任立军%郭峰
張成雷%孫清慧%任立軍%郭峰
장성뢰%손청혜%임립군%곽봉
还原型谷胱甘肽%重症急性胰腺炎%肺损伤%TNF-α%LIF
還原型穀胱甘肽%重癥急性胰腺炎%肺損傷%TNF-α%LIF
환원형곡광감태%중증급성이선염%폐손상%TNF-α%LIF
GSH%Severe acute pancreatitis%Lung injury%TNF-α%LIF
目的 研究还原型谷胱甘肽对重症急性胰腺炎(SAP)大鼠肺损伤的保护作用;探讨SAP肺损伤的发病机制.方法 54只SD大鼠随机分为对照组、模型组和治疗组.采用十二指肠乳头逆行插管胰胆管注射5%的牛磺胆酸钠的方法复制大鼠SAP模型,各组大鼠分别于建模后3h、6 h、12h剖杀,检测血清淀粉酶、肺含水量、肺组织中TNF-α、LIF基因的表达水平.结果 治疗组各时间点血清淀粉酶均低于模型组(P<0.05),但仍高于对照组(P<0.05);治疗组较模型组肺含水量减少(P<0.05),明显较对照组高(P<0.05);对照组肺组织中未见明显的LIF基因表达,建摸后3h、6 h、12h治疗组TNF-α、LIF基因的表达水平均低于模型组(P<0.05),模型组及治疗组大鼠肺组织中TNF-α基因的表达水平均高于对照组(P<0.05).结论 还原型谷胱甘肽对SAP大鼠肺损伤具有保护作用.
目的 研究還原型穀胱甘肽對重癥急性胰腺炎(SAP)大鼠肺損傷的保護作用;探討SAP肺損傷的髮病機製.方法 54隻SD大鼠隨機分為對照組、模型組和治療組.採用十二指腸乳頭逆行插管胰膽管註射5%的牛磺膽痠鈉的方法複製大鼠SAP模型,各組大鼠分彆于建模後3h、6 h、12h剖殺,檢測血清澱粉酶、肺含水量、肺組織中TNF-α、LIF基因的錶達水平.結果 治療組各時間點血清澱粉酶均低于模型組(P<0.05),但仍高于對照組(P<0.05);治療組較模型組肺含水量減少(P<0.05),明顯較對照組高(P<0.05);對照組肺組織中未見明顯的LIF基因錶達,建摸後3h、6 h、12h治療組TNF-α、LIF基因的錶達水平均低于模型組(P<0.05),模型組及治療組大鼠肺組織中TNF-α基因的錶達水平均高于對照組(P<0.05).結論 還原型穀胱甘肽對SAP大鼠肺損傷具有保護作用.
목적 연구환원형곡광감태대중증급성이선염(SAP)대서폐손상적보호작용;탐토SAP폐손상적발병궤제.방법 54지SD대서수궤분위대조조、모형조화치료조.채용십이지장유두역행삽관이담관주사5%적우광담산납적방법복제대서SAP모형,각조대서분별우건모후3h、6 h、12h부살,검측혈청정분매、폐함수량、폐조직중TNF-α、LIF기인적표체수평.결과 치료조각시간점혈청정분매균저우모형조(P<0.05),단잉고우대조조(P<0.05);치료조교모형조폐함수량감소(P<0.05),명현교대조조고(P<0.05);대조조폐조직중미견명현적LIF기인표체,건모후3h、6 h、12h치료조TNF-α、LIF기인적표체수평균저우모형조(P<0.05),모형조급치료조대서폐조직중TNF-α기인적표체수평균고우대조조(P<0.05).결론 환원형곡광감태대SAP대서폐손상구유보호작용.
Objective To investigate the protective effect of GSH on lung injury in rats with severe acute pancreatitis(SAP) and investigate the mechanism of severe acute pancreatitis associated lung injury. Methods Fifty-four Spragwe Dawley rats were randomly divided into three groups: control group, model(SAP)group and herbal group. Rats in model group and herbal group were induced pancreatitis by retrograde injection of 5% sodium taurocholate into the pancreatobiliary duct. Animals were killed at 3h, 6h, 12h after injection. The serum amylase was examined at 3h, 6h, 12h after injection. Pulmonary tissue fluid content was measured.The expression of pulmonary TNF-α mRNA and LIF mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Results The serum level of amylase was lowered significantly in herbal group in comparision with that in model group (P<0.05) and pulmonary tissue fluid content in herbal group was reduced compared with model group (P<0.05). Similarly, the level of pulmonary TNF-α mRNA and LIF mRNA expressions was both significantly reduced in herbal group compared with model group (P<0.05). LIF mRNA was not examined in normal control group. Conclusion Treatment with GSH can prevent lung injury of rats with SAP.
