中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
1期
64-68
,共5页
王海岩%李岩%徐爱强%张勇%陶泽新%刘桂芳%刘尧%宋立志%张丽%颜丙玉%纪峰%许文波
王海巖%李巖%徐愛彊%張勇%陶澤新%劉桂芳%劉堯%宋立誌%張麗%顏丙玉%紀峰%許文波
왕해암%리암%서애강%장용%도택신%류계방%류요%송립지%장려%안병옥%기봉%허문파
柯萨奇B5病毒%无菌性脑膜炎%亲缘进化分析
柯薩奇B5病毒%無菌性腦膜炎%親緣進化分析
가살기B5병독%무균성뇌막염%친연진화분석
Coxsackie-virus B5%Aseptic meningitis%Phylogenic analysis
目的 鉴定引起2005年山东省兖州市一起无菌性脑膜炎暴发的病原体,并对分离的柯萨奇B5病毒(CVB5)进行基因特征分析.方法 从兖州市无菌性脑膜炎暴发期间部分住院患者中采集78份粪便标本和58份脑脊液标本进行病毒分离和血清型鉴定;同时对其阳性分离物采用反转录-聚合酶链反应进行分子定型,对其中的CVB5进行全长VP1区核苷酸序列测定和亲缘进化分析.结果 78份粪便标本和58份脑脊液标本的病毒分离率分别为38.5%(30/78)和48.3%(28/58).58株阳性分离物的血清型鉴定和分子定型结果显示,其中54株为CVB5,2株为ECHO24,CVB3和CVA9各1株.病原学结果显示引起此次无菌性脑膜炎暴发的病原体主要为CVB5.同源性分析显示,CVB5分离株与2002年浙江分离株(Zhejiang/12/02株)在核苷酸水平上同源性最近,为97.5%～97.8%;与CVB5原型株(Faulkner株)相比核苷酸同源性为78.3%～78.6%.VP1区亲缘进化树显示CVB5可以分为A、B、C、D 4个基因型,引起本次暴发的CVB5属于基因型D.结论 CVB5是引起兖州市无菌性脑膜炎暴发的病原体.优势基因型的变迁可能是造成无菌性脑膜炎反复暴发的因素之一.
目的 鑒定引起2005年山東省兗州市一起無菌性腦膜炎暴髮的病原體,併對分離的柯薩奇B5病毒(CVB5)進行基因特徵分析.方法 從兗州市無菌性腦膜炎暴髮期間部分住院患者中採集78份糞便標本和58份腦脊液標本進行病毒分離和血清型鑒定;同時對其暘性分離物採用反轉錄-聚閤酶鏈反應進行分子定型,對其中的CVB5進行全長VP1區覈苷痠序列測定和親緣進化分析.結果 78份糞便標本和58份腦脊液標本的病毒分離率分彆為38.5%(30/78)和48.3%(28/58).58株暘性分離物的血清型鑒定和分子定型結果顯示,其中54株為CVB5,2株為ECHO24,CVB3和CVA9各1株.病原學結果顯示引起此次無菌性腦膜炎暴髮的病原體主要為CVB5.同源性分析顯示,CVB5分離株與2002年浙江分離株(Zhejiang/12/02株)在覈苷痠水平上同源性最近,為97.5%～97.8%;與CVB5原型株(Faulkner株)相比覈苷痠同源性為78.3%～78.6%.VP1區親緣進化樹顯示CVB5可以分為A、B、C、D 4箇基因型,引起本次暴髮的CVB5屬于基因型D.結論 CVB5是引起兗州市無菌性腦膜炎暴髮的病原體.優勢基因型的變遷可能是造成無菌性腦膜炎反複暴髮的因素之一.
목적 감정인기2005년산동성연주시일기무균성뇌막염폭발적병원체,병대분리적가살기B5병독(CVB5)진행기인특정분석.방법 종연주시무균성뇌막염폭발기간부분주원환자중채집78빈분편표본화58빈뇌척액표본진행병독분리화혈청형감정;동시대기양성분리물채용반전록-취합매련반응진행분자정형,대기중적CVB5진행전장VP1구핵감산서렬측정화친연진화분석.결과 78빈분편표본화58빈뇌척액표본적병독분리솔분별위38.5%(30/78)화48.3%(28/58).58주양성분리물적혈청형감정화분자정형결과현시,기중54주위CVB5,2주위ECHO24,CVB3화CVA9각1주.병원학결과현시인기차차무균성뇌막염폭발적병원체주요위CVB5.동원성분석현시,CVB5분리주여2002년절강분리주(Zhejiang/12/02주)재핵감산수평상동원성최근,위97.5%～97.8%;여CVB5원형주(Faulkner주)상비핵감산동원성위78.3%～78.6%.VP1구친연진화수현시CVB5가이분위A、B、C、D 4개기인형,인기본차폭발적CVB5속우기인형D.결론 CVB5시인기연주시무균성뇌막염폭발적병원체.우세기인형적변천가능시조성무균성뇌막염반복폭발적인소지일.
Objective To identify the pathogen that caused an outbreak of aseptic meningitis in Shandong province in 2005. Phylogenic analysis was carried out on Coxsackie-virus B5 (CVB5) which was isolated during this outbreak. Methods 78 stool and 58 cerebrospinal fluids (CSF) specimens were collected from some inpatients during this outbreak. Virus isolation and reverse transcription-polymerase chain reaction (RT-PCR) was then performed. Phylo-genetic trees based on entire and partial VP1 sequences were constructed among CVB5 isolates and others published in GenBank. Results The isolation rates of stool and CSF specimens were 38.5% (30/78) and 48.3% (28/58) respectively. Among the results of serotype identification and molecular typing of 58 positive isolates, 54 were identified as CVB5, 2 as ECHO24, 1 as CVB3 and 1 as CVA9. Results from viral investigation showed that CVB5 was the main pathogen causing this outbreak. Data from homological comparisons indicated that Shandong strains had the highest nucleotide acid identity with the Zhejiang/ 12/02 strain (97.5%-97.8%), and lower identity (78.3%-78.6%) with the prototype strain (Faulkner strain). Phylogenic tree in VP1 region showed that CVB5 could be separated into four genotypes. Isolates of this outbreak belonged to genotype D. Conclusion CVB5 was the major etiological agent correlated with this outbreak. The shift of predominant genotype might serve as one of the causes that associated with the outbreaks of aseptic meningitis.
