CAJ | 학술논문

目的了解浙江沿海地区人群TTV与HPV混合感染状况,探讨TTV传播途径.方法建立巢式聚合酶链反应方法(nPCR),对健康体检和患宫颈疾病妇女150例宫颈病变细胞学标本及其平行97例血清标本进行TTV DNA及TTV病毒滴度的nPCR检测;应用导流杂交方法检测95例宫颈病变细胞学标本的HPV基因型.结果 TTV DNA在55例健康妇女宫颈细胞标本中检出率52.7%(29/55),与其平行42例血清样本中检出率50.0%(21/42).在患有疾病妇女宫颈细胞中TTV DNA检出率(74.7%)高于健康体检对照组(P=0.005).TTV DNA在患者血清样本中检出率51%(28/55).在宫颈细胞及其平行血清中均检测出TTV基因亚型G1b°TTV病毒滴度在宫颈细胞中高于在其平行血清10～1000倍.HPV在患者组中检出率98.9%(94/95),在健康组中检出率27.3%(15/55).HPV基因型是高危型HPV16、18、33和低危型HPV6.HPV阳性标本TTV DNA检测率明显高于HPV阴性标本(P=0.02).结论 TTV在宫颈细胞中具有高检出率,在宫颈细胞中TTV病毒滴度高于其平行血清.TTV与HPV随性传播感染人群,并在女性生殖道内繁殖.TTV与HPV协同作用有待研究.
목적 료해절강연해지구인군TTV여HPV혼합감염상황,탐토TTV전파도경.방법 건립소식취합매련반응방법(nPCR),대건강체검화환궁경질병부녀150례궁경병변세포학표본급기평행97례혈청표본진행TTV DNA급TTV병독적도적nPCR검측;응용도류잡교방법검측95례궁경병변세포학표본적HPV기인형.결과 TTV DNA재55례건강부녀궁경세포표본중검출솔52.7%(29/55),여기평행42례혈청양본중검출솔50.0%(21/42).재환유질병부녀궁경세포중TTV DNA검출솔(74.7%)고우건강체검대조조(P=0.005).TTV DNA재환자혈청양본중검출솔51%(28/55).재궁경세포급기평행혈청중균검측출TTV기인아형G1b°TTV병독적도재궁경세포중고우재기평행혈청10～1000배.HPV재환자조중검출솔98.9%(94/95),재건강조중검출솔27.3%(15/55).HPV기인형시고위형HPV16、18、33화저위형HPV6.HPV양성표본TTV DNA검측솔명현고우HPV음성표본(P=0.02).결론 TTV재궁경세포중구유고검출솔,재궁경세포중TTV병독적도고우기평행혈청.TTV여HPV수성전파감염인군,병재녀성생식도내번식.TTV여HPV협동작용유대연구.
Objective To investigate the prevalence of transfusion-transmitted virus (TTV) and human papillomavirus (HPV) co-infection in cervical smears of patients with cervical lesions in littoral of Zhejiang province and analysis of transmitted route.Methods Nested polymerase chain reaction (nPCR) was established.TTV DNA were tested by nPCR in cervical smears of 95 patients with cervical lesions and 55 healthy women,paired serum samples were available from 55 and 42 women,and their viral titer.The genotypes of 95 specimens of cervical cytology were detected with HybriMax.The phylogenetic group of TTV was determined by means of nPCR with N22 primers.Results The prevalence of TTV DNA in cervical smears of patients with cervical lesions and healthy women was 52.7% (29/55) and was comparable with that in paired serum sample (50%).Symptomatic women had significantly higher prevalence of TTV DNA in cervical smears (74.7%)than healthy controls (P=0.005).The TTV DNA prevalence in patient serum samples was 51%.The phyiogenetic groups of TTV serum isolates were concordant with those of TTV from cervical smears of the same subjects,and genotype was G1b.The TTV viral titer in cervical smears were 10 to 1000 times as high as in serum.The total infection rate of HPV was 98.9% in patients,and was 27.3% in healthy women.The frequently detected genotype was HPV16,18,33 of HSIL,and HPV6 of LSIL.The HPV positive study subjects had significantly higher TTV DNA prevalence than HPV negatives (P=0.02).Conclusion High prevalence of TTV in cervical smears suggests that sexual transmission is another mode of expansion of TTV infection among the population.The higher viral titer in cervical smears than in the respective serum samples might indicate active TTV replication in the female genital tract.Nevertheless,cooperation between TTV and HPV needs to be further investigated.