中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2012年
2期
129-132
,共4页
罗中捷%周卓晟%郝春慧%郭寅生%刘红艳%郑华英%黄正
囉中捷%週卓晟%郝春慧%郭寅生%劉紅豔%鄭華英%黃正
라중첩%주탁성%학춘혜%곽인생%류홍염%정화영%황정
大肠杆菌O157%生态学%酶联免疫吸附测定%活的非可培养态%微生态
大腸桿菌O157%生態學%酶聯免疫吸附測定%活的非可培養態%微生態
대장간균O157%생태학%매련면역흡부측정%활적비가배양태%미생태
Escherichia coli O157%Ecology%Enzyne-linked immunosorbent assay%Viable but noncuhurable%Microcosm
目的 监测大肠杆菌O157∶H7在微生态汉江水中活的非可培养态；探讨荧光染色、异养平板计数和ELISA检测活的非可培养态大肠杆菌O157∶H7的可行性.方法 将大肠杆菌O157∶H7投加到模拟微生态汉江水样中,通过改变温度和营养条件,分别采用吖啶橙染色、活菌直接计数-5-氰基-2,3-二-(P-苄基-四唑氯化物)染色、活菌直接计数-萘啶酮酸染色、异养平板计数和ELISA法实时监测大肠杆菌O157∶H7的存活情况.结果 在4℃、寡营养条件下,大肠杆菌O157∶H7在汉江水中存活58 d后进入活的非可培养态,此时采用活菌直接计数-5-氰基-2,3-二-(P-苄基-四唑氯化物)染色法测得活菌数为1.2×105 CFU/ml,活菌直接计数-萘啶酮酸染色法测得活菌数为9.0×104 CFU/ml,异养平板计数法测得可培养菌数为0,ELISA法测得微生态汉江水中大肠杆菌O157∶H7总菌数在58 d内基本稳定于106 CFU/ml的初始菌数水平.结论 在4℃、寡营养条件下,大肠杆菌O157∶H7在微生态汉江水中可进入活的非可培养态；ELISA结合荧光染色、异养平板计数,能定量检出活的非可培养态大肠杆菌O157∶H7.
目的 鑑測大腸桿菌O157∶H7在微生態漢江水中活的非可培養態；探討熒光染色、異養平闆計數和ELISA檢測活的非可培養態大腸桿菌O157∶H7的可行性.方法 將大腸桿菌O157∶H7投加到模擬微生態漢江水樣中,通過改變溫度和營養條件,分彆採用吖啶橙染色、活菌直接計數-5-氰基-2,3-二-(P-芐基-四唑氯化物)染色、活菌直接計數-萘啶酮痠染色、異養平闆計數和ELISA法實時鑑測大腸桿菌O157∶H7的存活情況.結果 在4℃、寡營養條件下,大腸桿菌O157∶H7在漢江水中存活58 d後進入活的非可培養態,此時採用活菌直接計數-5-氰基-2,3-二-(P-芐基-四唑氯化物)染色法測得活菌數為1.2×105 CFU/ml,活菌直接計數-萘啶酮痠染色法測得活菌數為9.0×104 CFU/ml,異養平闆計數法測得可培養菌數為0,ELISA法測得微生態漢江水中大腸桿菌O157∶H7總菌數在58 d內基本穩定于106 CFU/ml的初始菌數水平.結論 在4℃、寡營養條件下,大腸桿菌O157∶H7在微生態漢江水中可進入活的非可培養態；ELISA結閤熒光染色、異養平闆計數,能定量檢齣活的非可培養態大腸桿菌O157∶H7.
목적 감측대장간균O157∶H7재미생태한강수중활적비가배양태；탐토형광염색、이양평판계수화ELISA검측활적비가배양태대장간균O157∶H7적가행성.방법 장대장간균O157∶H7투가도모의미생태한강수양중,통과개변온도화영양조건,분별채용아정등염색、활균직접계수-5-청기-2,3-이-(P-변기-사서록화물)염색、활균직접계수-내정동산염색、이양평판계수화ELISA법실시감측대장간균O157∶H7적존활정황.결과 재4℃、과영양조건하,대장간균O157∶H7재한강수중존활58 d후진입활적비가배양태,차시채용활균직접계수-5-청기-2,3-이-(P-변기-사서록화물)염색법측득활균수위1.2×105 CFU/ml,활균직접계수-내정동산염색법측득활균수위9.0×104 CFU/ml,이양평판계수법측득가배양균수위0,ELISA법측득미생태한강수중대장간균O157∶H7총균수재58 d내기본은정우106 CFU/ml적초시균수수평.결론 재4℃、과영양조건하,대장간균O157∶H7재미생태한강수중가진입활적비가배양태；ELISA결합형광염색、이양평판계수,능정량검출활적비가배양태대장간균O157∶H7.
Objectives This study is to monitor the survival of E.coli O157∶H7 in the aquatic microcosm from Han River and explore the feasibility of fluorescence staining,heterotrophic plate count and ELISA in detection of viable but nonculturable E.coli O157∶ H7.Methods E.coli O157∶H7 were added into aquatic microcosm from Han River and cultured with low temperature and olig-nutrition.Then the survival of E.coli O157∶H7 were real-time monitored by acridine orange direct count(AODC),direct viable count(DVC)-CTC staining,DVC-nalidixic acid(NA)staining,heterotrophic plate count(HPC)and ELISA.Results E.coli O157∶H7 can be converted to a viable but nonculturable state in the aquatic microcosm from Han River 58 days after cultured at 4 ℃ with oligo-nutrition.The amount of viable E.coli O157∶H7 was measured as 1.2 × 105 CFU/ml by DVC-CTC and 9.0 × 104 CFU/ml by DVC-NA,whereas the amount of culturable bacterial determined by HPC is 0.The amounts of bacteria determined by ELISA are basically stable within 58 days around 106 CFU/ml.Conclusion E.coli O157∶H7 can be converted into a viable but nonculturable state in Han River water at 4 ℃ with oligo-nutrition,and ELISA combined with fluorescence staining and heterotrophic plate count can be used in quantitative detection of the viable but nonculturable E.coli O157∶ H7.
