中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2009年
5期
365-368
,共4页
刘延山%陈刚%刘毅%李蕊%王之奇%申岱
劉延山%陳剛%劉毅%李蕊%王之奇%申岱
류연산%진강%류의%리예%왕지기%신대
骨生成%牵张%腭裂%骨桥蛋白%骨钙蛋白
骨生成%牽張%腭裂%骨橋蛋白%骨鈣蛋白
골생성%견장%악렬%골교단백%골개단백
Osteogenesis%distraction%Cleft palate%Osteopetin%Osteocalcin
目的 以牵张成骨术整复猕猴腭裂骨缺损,定量分析新骨在不同时期骨桥蛋白(osteopetin,OPN)与骨钙蛋白(osteocalcin,OC)的表达水平,探讨新骨生成与改建的规律.方法 以猕猴为对象建立腭裂动物模型.实验组动物21只行牵张成骨术整复其聘部软硬组织缺损,关闭裂隙后固定.固定期第1、2 4、6、8、12及24周分别取材,各3只动物.采用实时定量PeR法(real-time,RT-PCR)定量比较OPN与OC的mRNA表达水平,并以酶联免疫吸附试验法(ELISA)定量分析其OPN与OC含量,与实验对照组及健康对照组(各2只动物)结果进行比较.结果 固定期第2周OPNmRNA表达上调,第4周达最高(7.59±0.37);而OC mRNA表达则自第4周开始上调(4.98±0.21),第6周时达最大值(7.94±0.31);随后开始下降,至第24周时两者的mRNA表达水平接近健康对照组(P>0.05).ELISA结果显示:固定期第4、6周OPN分别为(4.75±0.15)ng/mg和(4.86±0.09)ng/mg,OC分别为(3.18±0.16)ng/mg和(3.63±0.33)ng/mg,两者均为高水平表达.至第8～12周以后蛋白表达趋势与其对应mRNA表达基本一致.结论 应用牵张成骨术整复腭裂骨缺损,其牵张区域新骨生成,裂隙被骨运送盘移动封闭,腭部裂隙被完全修复.
目的 以牽張成骨術整複獼猴腭裂骨缺損,定量分析新骨在不同時期骨橋蛋白(osteopetin,OPN)與骨鈣蛋白(osteocalcin,OC)的錶達水平,探討新骨生成與改建的規律.方法 以獼猴為對象建立腭裂動物模型.實驗組動物21隻行牽張成骨術整複其聘部軟硬組織缺損,關閉裂隙後固定.固定期第1、2 4、6、8、12及24週分彆取材,各3隻動物.採用實時定量PeR法(real-time,RT-PCR)定量比較OPN與OC的mRNA錶達水平,併以酶聯免疫吸附試驗法(ELISA)定量分析其OPN與OC含量,與實驗對照組及健康對照組(各2隻動物)結果進行比較.結果 固定期第2週OPNmRNA錶達上調,第4週達最高(7.59±0.37);而OC mRNA錶達則自第4週開始上調(4.98±0.21),第6週時達最大值(7.94±0.31);隨後開始下降,至第24週時兩者的mRNA錶達水平接近健康對照組(P>0.05).ELISA結果顯示:固定期第4、6週OPN分彆為(4.75±0.15)ng/mg和(4.86±0.09)ng/mg,OC分彆為(3.18±0.16)ng/mg和(3.63±0.33)ng/mg,兩者均為高水平錶達.至第8～12週以後蛋白錶達趨勢與其對應mRNA錶達基本一緻.結論 應用牽張成骨術整複腭裂骨缺損,其牽張區域新骨生成,裂隙被骨運送盤移動封閉,腭部裂隙被完全脩複.
목적 이견장성골술정복미후악렬골결손,정량분석신골재불동시기골교단백(osteopetin,OPN)여골개단백(osteocalcin,OC)적표체수평,탐토신골생성여개건적규률.방법 이미후위대상건립악렬동물모형.실험조동물21지행견장성골술정복기빙부연경조직결손,관폐렬극후고정.고정기제1、2 4、6、8、12급24주분별취재,각3지동물.채용실시정량PeR법(real-time,RT-PCR)정량비교OPN여OC적mRNA표체수평,병이매련면역흡부시험법(ELISA)정량분석기OPN여OC함량,여실험대조조급건강대조조(각2지동물)결과진행비교.결과 고정기제2주OPNmRNA표체상조,제4주체최고(7.59±0.37);이OC mRNA표체칙자제4주개시상조(4.98±0.21),제6주시체최대치(7.94±0.31);수후개시하강,지제24주시량자적mRNA표체수평접근건강대조조(P>0.05).ELISA결과현시:고정기제4、6주OPN분별위(4.75±0.15)ng/mg화(4.86±0.09)ng/mg,OC분별위(3.18±0.16)ng/mg화(3.63±0.33)ng/mg,량자균위고수평표체.지제8～12주이후단백표체추세여기대응mRNA표체기본일치.결론 응용견장성골술정복악렬골결손,기견장구역신골생성,렬극피골운송반이동봉폐,악부렬극피완전수복.
Objective To study the mechanism of new bone formation and remodeling of distraction osteogenesis(DO) by analysis of the expression of osteopotin(OPN)and osteecalcin(OC). Methods Rhesus were operated to reconstruct the animal model of cleft palate(CP). The CP was closed by DO in experimental group(n=21). After consolidation of 1, 2, 4, 6, 8, 12, 24 weeks, every 3 animals were killed to collect the specimens, respectively. The OPN and OC and their mRNA were detected quantitatively by Real-time RT-PCR and ELISA, respectively. The animals in control group(n=2) and sham group(n=2) were used as control. Results The mRNA expression of OPN increased since 2nd week of consolidation and reached the peak at 4th week(7.59±0.37). The mRNA expression of OC was up-regulaed since 4th week, and reach the peak at 6th week(7.94±0.31). Then they decreased to about the level in sham group at 24th week(P > 0.05). The OPN and OC were highly expressed during 4 to 6 weeks of consolidation. During 8 to 12 weeks, they decreased like their mRNA expression. Conclusion The intramembraneons new bone formation after DO can reconstruct the bone defect of CP. The new formed bone can be remodeled to be quite normal bone tissue.
