CAJ | 학술논문

背景:弱激光的生物刺激作用已被许多实验研究及临床应用证实,其作用表现在促进组织修复、镇痛、抗炎等方面.目的:观察He-Ne激光和小剂量CO2激光照射对骨折愈合的作用.设计:随机对照动物实验.单位:承德医学院激光医学研究室.材料:选择健康雄性新西兰白兔48只,体质量2.0～2.5 kg.方法:实验于1998/2003在承德医学院激光医学研究室完成.①将48只新西兰白兔左桡骨造成实验性骨折,将之分为3组,每组16只,其中一组为对照组,另外两组分别接受28 mW/cm2的He-Ne激光照射(He-Ne组)及150 mW/cm2的CO2激光照射(CO2组),1次/d,10 min/次,直接照射骨折部位.②骨折后15 d和30 d拍摄X射线片后分批处死动物.取出实验骨,对15 d的实验骨,测定骨痂中的钙与胶原含量.对35 d的实验骨,测定生物力学抗扭性能.主要观察指标:①各组X射线片观察结果.②各组骨痂中胶原与钙的含量.③各组生物力学抗扭强度测试结果.结果:45只进入结果分析.①骨折后15 d,CO2组及He-Ne组的X射线片、骨痂中的胶原及钙含量均优于对照组,且CO2组骨痂中的胶原及钙含量高于He-Ne组[(341.9±30.1)比(302.1±28.7)mg/g,(197.1±19.7)比(156.5±17.6)mg/g,P＜0.05].②骨折后35 d,CO2激光及He-Ne激光照射组的生物力学抗扭性能均优于对照组,两个照射组之间的差异无显著性.③两个时间得到的X射线片的结果显示CO2激光及He-Ne激光照射组均优于对照组,两个照射组之间的差异无显著性.结论:CO2激光及He-Ne激光照射均促进了骨折愈合,且在骨折后15 d时,CO2激光照射的作用效果显著优于He-Ne激光照射,而在骨折后35 d时,从临床意义上讲,其差异无显著性.
배경:약격광적생물자격작용이피허다실험연구급림상응용증실,기작용표현재촉진조직수복、진통、항염등방면.목적:관찰He-Ne격광화소제량CO2격광조사대골절유합적작용.설계:수궤대조동물실험.단위:승덕의학원격광의학연구실.재료:선택건강웅성신서란백토48지,체질량2.0～2.5 kg.방법:실험우1998/2003재승덕의학원격광의학연구실완성.①장48지신서란백토좌뇨골조성실험성골절,장지분위3조,매조16지,기중일조위대조조,령외량조분별접수28 mW/cm2적He-Ne격광조사(He-Ne조)급150 mW/cm2적CO2격광조사(CO2조),1차/d,10 min/차,직접조사골절부위.②골절후15 d화30 d박섭X사선편후분비처사동물.취출실험골,대15 d적실험골,측정골가중적개여효원함량.대35 d적실험골,측정생물역학항뉴성능.주요관찰지표:①각조X사선편관찰결과.②각조골가중효원여개적함량.③각조생물역학항뉴강도측시결과.결과:45지진입결과분석.①골절후15 d,CO2조급He-Ne조적X사선편、골가중적효원급개함량균우우대조조,차CO2조골가중적효원급개함량고우He-Ne조[(341.9±30.1)비(302.1±28.7)mg/g,(197.1±19.7)비(156.5±17.6)mg/g,P＜0.05].②골절후35 d,CO2격광급He-Ne격광조사조적생물역학항뉴성능균우우대조조,량개조사조지간적차이무현저성.③량개시간득도적X사선편적결과현시CO2격광급He-Ne격광조사조균우우대조조,량개조사조지간적차이무현저성.결론:CO2격광급He-Ne격광조사균촉진료골절유합,차재골절후15 d시,CO2격광조사적작용효과현저우우He-Ne격광조사,이재골절후35 d시,종림상의의상강,기차이무현저성.
BACKGROUND: The effects of low-level laser biostimulation have been proved by various experimental studies and clinical application, which are manifested as tissue repairing, analgesia, antiinflammation, etc.OBJECTIVE: To observe the effects of He-Ne laser and low-level CO2 laser irradiation on fracture healing.DESIGN: A randomized controlled animal experiment. SETTING: Research Room of Laser Medicine, Chengde Medical College.MATERIALS: Forty-eight healthy male New Zealand rabbits, weighing 2.0-2.5 kg, were used.METHODS: The experiments were carried out in the Research Room of Laser Medicine, Chengde Medical College from 1998 to 2003. ①The 48 rabbits were induced into models of experimental fracture of the left radius, and then they were divided into three groups with 16 rabbits in each group: control group, He-Ne laser irradiation group and CO2 laser irradiation group. The fracture areas of the animals in the He-Ne and CO2 laser irradiation groups were respectively irradiated with 28 mW/cm2 He-Ne laser and 150 mW/cm2 CO2 laser for 10 minutes, once a day. ② The animals were killed on the 15th and 35th days after taking roentgenogram respectively. At 15 days, the collagen and calcium contents in callus were detected. At 35 days, the biomechanic anti-torsion strength was examined.MAIN OUTCOME MEASURES: ①Results of roentgenogram in each group; ② Collagen and calcium contents in callus; ③ Results of biomechanic anti-torsion strength.RESULTS: Totally 45 rabbits were involved in the analysis of results. ① On the 15th and day after fracture, the roentgenogram, collagen and calcium contents in callus in the He-Ne and CO2 laser irradiation groups were better than those in the control group, and the collagen and calcium contents in callus were higher in the CO2 laser irradiation group than in the He-Ne laser irradiation group [(341.9±30.1) vs (302.1±28.7) mg/g; (197.1±19.7)vs (156.5±17.6) mg/g, P＜ 0.05]. ②On the 35th days after fracture, the biomechanic anti-torsion functions in the He-Ne and CO2 laser irradiation groups were superior to that in the control group, and there were no significant difference between the two laser irradiation groups. ③ The results of roentgenogram in the He-Ne and CO2 laser irradiation groups were better than that in the control group both on the 15th and 35th days after fracture, and there was no significant difference between the two irradiation groups.CONCLUSION: Both CO2 laser and He-Ne laser irradiations can accelerate the process of fracture healing, and the curative effect of CO2 laser is better than that of He-Ne laser on the 15th day after fracture, but there is no significant difference clinically between the CO2 laser and the He-Ne laser irradiations on the 35th day after fracture.