CAJ | 학술논문

背景:自制碳化硅陶瓷应用于临床前对其安全性进行相应评价.目的:通过微核实验检测自制碳化硅陶瓷对小鼠的致畸作用以评价其生物相容性.设计、时间及地点:随机对照动物实验,于2007-06/12在中国医科大学中心实验室完成.材料:N1H纯系小鼠80只按性别,体质量随机分成8组,每组10只:碳化硅0.02,0.002,0.000 2 mL/kg组、纯钛浸提液0.02,0.002,0.000 2 mL/kg组,生理盐水对照组,75 mg/kg环磷酰胺阳性对照组.方法:各组均一次性给药,24 h处死小鼠,抽取双侧股骨骨髓制备细胞悬液,推片二张,晾干、固定后浸入姬姆萨染液中染色.选择细胞完整.分散均匀,着色适当的区域在光学显微镜下计数.主要观察指标:计数1 000个嗜多染红细胞的微核细胞数;嗜多染红细胞与正红细胞的比值.结果:环磷酰胺对照组微核率高于其他组(p＜0.01).碳化硅陶瓷组和纯钛浸提液组微核率与生理盐水对照组比较,差异无显著性意义(p＞0.05).碳化硅陶瓷组与纯钛浸提液组比较差异无显著性意义,嗜多染红细胞与正红细胞的比值均≥1.结论:自制碳化硅对小鼠无致畸作用,有较良好的生物相容性.
배경:자제탄화규도자응용우림상전대기안전성진행상응평개.목적:통과미핵실험검측자제탄화규도자대소서적치기작용이평개기생물상용성.설계、시간급지점:수궤대조동물실험,우2007-06/12재중국의과대학중심실험실완성.재료:N1H순계소서80지안성별,체질량수궤분성8조,매조10지:탄화규0.02,0.002,0.000 2 mL/kg조、순태침제액0.02,0.002,0.000 2 mL/kg조,생리염수대조조,75 mg/kg배린선알양성대조조.방법:각조균일차성급약,24 h처사소서,추취쌍측고골골수제비세포현액,추편이장,량간、고정후침입희모살염액중염색.선택세포완정.분산균균,착색괄당적구역재광학현미경하계수.주요관찰지표:계수1 000개기다염홍세포적미핵세포수;기다염홍세포여정홍세포적비치.결과:배린선알대조조미핵솔고우기타조(p＜0.01).탄화규도자조화순태침제액조미핵솔여생리염수대조조비교,차이무현저성의의(p＞0.05).탄화규도자조여순태침제액조비교차이무현저성의의,기다염홍세포여정홍세포적비치균≥1.결론:자제탄화규대소서무치기작용,유교량호적생물상용성.
BACKGROUND:Safety of self-made silicon carbide ceramic(SiC)was evaluated before clinical application.OBJECTIVE:To study the biocornpatibility of low-Ni non-Be SiC alloy,detect its teratogenic effect to the mice with micronuclei test.DESIGN,TIME AND SETTING:Randomized controlled animal study was performed at Central Laboratory of China Medical Universitv from June tO December 2007.MATERIALS:86 mice were randomly divided into 8 groups by sex and body mass,including SiC(0.02,0.002,0.000 2 mL/kg)groups,Ni-Cr-Ni extract(0.02,0.002,0.000 2 mL/kg)groups,saline group,and 75 mg/kgcyclophosphamide group.with 10 mice in each group.METHODS:Mice in each group were administrated once and sacrificed after 24 hours.Bone marrow extracted from bilateral femur was used to make cell suspension.Slide specimens were fixed,dried,and dipped into Giemsa,The district which had complete cell,well-distributed,and well-coloration,was selected and counted under light mlcroscope,MAIN OUTCOME MEASURES:1000 micronucleated cells of polychromatic erythro-cytes(MNPCE)were counted,and ratio between polychromatic erythro-cytes(PCE)and normochromatic erythrocytes(NRBC),i.e.,P/N,wascalculated.RESULTS:Micronuclear rates in the cyclophosphamide group were higher than other groups(P＜0.01):however,there wete no significant differences in micronuclear rates among SiC ceramic group,Ni-Cr-Ni extract group,and saline group(P＞0.05);while,there were also no significant differences between SiC ceramic group and Ni-Cr-Ni extract grouP.The P/N value was≥1.CONCLUSION:The self-made SiC has no teratogenic effect to mice and shows great biocompatibility