CAJ | 학술논문

目的观察不同能流密度体外冲击波对骨质疏松兔股骨髁部松质骨成骨作用的差异.方法采用卵巢切除法(OVX)对30只5月龄雌性新西兰兔去势,5个月后建立骨质疏松模型.将所有骨质疏松兔随机分为3组,每组10只,其中一组为空白对照组(A组),一组为体外冲击波(ESW)能流密度0.28 mJ/mm~2处理组(B组),另一组为体外冲击波能流密度0.47 mJ/mm~2处理组(C组),脉冲次数均为2000次,在实验组兔右侧股骨髁部进行体外冲击波(ESW)处理.于处理后4、8周时分二批每组处死5只动物,分离右侧股骨远端,进行显微CT(micro-CT)测量分析.结果 micro-CT三维重建分析表明,不同能流密度冲击波处理后4周时B组和C组在骨组织体积比(BV/TV)、骨小梁数目(Tb.N)等指标之间的差异有统计学意义;8周时B组和C组分别在骨密度(BMD)、骨矿含量(BMC)和BV/TV、骨表面积体积比(BS/BV)、骨小梁连接密度(Connectivity Density)等指标之间的差别有统计学意义,其中C组的BMD和BMC分别较B组增高16.8%和10.6%,尽管C组在其他骨小梁立体测量学等指标方面均较B组优,但两组测量结果的差别无统计学意义.结论不同能流密度体外冲击波对骨质疏松局部治疗后,不同时间,其成骨作用不同,较高能流密度的体外冲击波在治疗后8周时,促进骨质疏松被处理局部骨小梁的改建、改善骨小梁的三维结构、增加骨密度作用较优.
목적 관찰불동능류밀도체외충격파대골질소송토고골과부송질골성골작용적차이.방법 채용란소절제법(OVX)대30지5월령자성신서란토거세,5개월후건립골질소송모형.장소유골질소송토수궤분위3조,매조10지,기중일조위공백대조조(A조),일조위체외충격파(ESW)능류밀도0.28 mJ/mm~2처리조(B조),령일조위체외충격파능류밀도0.47 mJ/mm~2처리조(C조),맥충차수균위2000차,재실험조토우측고골과부진행체외충격파(ESW)처리.우처리후4、8주시분이비매조처사5지동물,분리우측고골원단,진행현미CT(micro-CT)측량분석.결과 micro-CT삼유중건분석표명,불동능류밀도충격파처리후4주시B조화C조재골조직체적비(BV/TV)、골소량수목(Tb.N)등지표지간적차이유통계학의의;8주시B조화C조분별재골밀도(BMD)、골광함량(BMC)화BV/TV、골표면적체적비(BS/BV)、골소량련접밀도(Connectivity Density)등지표지간적차별유통계학의의,기중C조적BMD화BMC분별교B조증고16.8%화10.6%,진관C조재기타골소량입체측량학등지표방면균교B조우,단량조측량결과 적차별무통계학의의.결론 불동능류밀도체외충격파대골질소송국부치료후,불동시간,기성골작용불동,교고능류밀도적체외충격파재치료후8주시,촉진골질소송피처리국부골소량적개건、개선골소량적삼유결구、증가골밀도작용교우.
Objective To investigate the difference of bone formation effects of different energy flux density extracorporeal shock wave on femoral condyle in osteoporosis rabbit in vivo.Methods Thirty New Zealand white rabbits were ovariectomied for 5 months to establish osteoporosis animal model. All animals were randomized into 3 groups: group A (sham), group B were used 0.28 mJ/mm~2 energy flux density extracorporeal shock wave and group C were used 0.47 mJ/mm~2, shock wave pulses are 2000. The right femoral condyles of all the test animal were applied. All animals were sacrificed after ESW treatment 4 or 8 weeks, and underwent micro-CT scan.Results Micro-CT analysis showed that bone volume/tissue volume(BV/TV) and trabecular number(Tb.N) have significant differences on 4 weeks after ESW treatment between the group B and C. On 8 weeks after ESW treatment, the group C, bone mineral density (BMD), bone mineral content (BMC), bone volume/tissue volume(BV/TV), bone surface/tissue volume (BS/TV) and trabecular connectivity density were higher significantly than those in group B, the BMD and BMC in group C were 16.8% and 10.6% higher than those in group B respectively. Although the other micro-architectural parameters in group C were higher than those in group B on 8 weeks after ESW treatment, but no significant differences were detected between the two groups.Conclusion In different time, different energy flux density extracorporeal shock wave used on osteoporosis animal made different effect. It is showed that higher energy flux density extracorporeal shock improve the reconstruction, remodeling of cancellous bone and increase the BMD is better than others on 8 weeks.