CAJ | 학술논문

YLZZ-2是一种具有降解苦马豆素活性的嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia).采用正交试验设计对影响其降解能力的培养基初始pH值、温度、底物浓度和摇床转速等4个因素进行优化,以对YLZZ-2的降解特性进行初步了解.结果表明:YLZZ-2菌在pH 7.0,温度30℃,SW添加浓度5%,转速160 r·min~(-1)时具有最佳降解SW能力.抗性检测表明,该菌具有氨苄青霉素、卡那霉素双重抗性,质粒消除后抗性消失,降解性也丧失,说明YLZZ-2菌降解SW的功能与质粒相关.
YLZZ-2시일충구유강해고마두소활성적기맥아과양단포균(Stenotrophomonas maltophilia).채용정교시험설계대영향기강해능력적배양기초시pH치、온도、저물농도화요상전속등4개인소진행우화,이대YLZZ-2적강해특성진행초보료해.결과표명:YLZZ-2균재pH 7.0,온도30℃,SW첨가농도5%,전속160 r·min~(-1)시구유최가강해SW능력.항성검측표명,해균구유안변청매소、잡나매소쌍중항성,질립소제후항성소실,강해성야상실,설명YLZZ-2균강해SW적공능여질립상관.
YLZZ-2 is a strain of Stenotrophomonas maltophilia capable of Swainsonine (SW) degradation. In this study, four condition factors were optimized by orthogonal test including initial culture medium pH, temperature, SW substrate concentration, and speed of shaking. The results show that YLZZ-2 could degrade SW at its best efficiency under the condition of pH value 7.0, temperature 30℃, SW concentration 5%, and shaking speed 160 r·min~(-1). Results of resistance test show that the bacteria had ampicillin-and-kanamycin resistance. The resistance disappeared and the ability of SW degradation lost when the plasmids were eliminated from YLZZ-2, which indicates that the SW degradation function of YLZZ-2 is associated with its plasmids.