CAJ | 학술논문

目的:探讨直肠癌远端移行黏膜COX-2及BCL-2蛋白的表达情况,判断直肠癌远端移行黏膜是否为癌前病变.方法:应用高铁二胺-阿辛蓝染色(HID/AB)检测54例直肠癌远端2 cm处黏膜,将远端黏膜分为移行黏膜(transitional mucosa,TM)组及非移行黏膜(non-transitional mucosa,NTM)组,通过免疫组织化学(s-p法)的方法检测两组黏膜组织、肿瘤组织以及20例手术切除的直肠良性息肉旁(cm)肠黏膜组织中COX-2蛋白和BCL-2蛋白的表达情况.比较TM内BCL-2以及COX-2的表达与其余各组的表达的差异.结果:54例直肠癌远端2cm处黏膜有移行黏膜19例,非移行黏膜35例.TM和NTM内均可检测到BCL-2及COX-2蛋白的表达,二者与肿瘤内BCL-2及COX-2蛋白的表达有显著差异(P<0.001),与直肠良性息肉旁肠黏膜组织中COX-2蛋白和BCL-2蛋白的表达的无差异(P>0.05),移行黏膜内COX-2、BCL-2蛋白的表达与非移行黏膜的表达的差别无统计学意义(P>0.05).结论:直肠癌远端TM内癌基因蛋白COX-2和BCL-2蛋白的表达无特异性,移行黏膜并非是癌前病变;直肠癌远端切除2 cm已足够.
목적:탐토직장암원단이행점막COX-2급BCL-2단백적표체정황,판단직장암원단이행점막시부위암전병변.방법:응용고철이알-아신람염색(HID/AB)검측54례직장암원단2 cm처점막,장원단점막분위이행점막(transitional mucosa,TM)조급비이행점막(non-transitional mucosa,NTM)조,통과면역조직화학(s-p법)적방법검측량조점막조직、종류조직이급20례수술절제적직장량성식육방(cm)장점막조직중COX-2단백화BCL-2단백적표체정황.비교TM내BCL-2이급COX-2적표체여기여각조적표체적차이.결과:54례직장암원단2cm처점막유이행점막19례,비이행점막35례.TM화NTM내균가검측도BCL-2급COX-2단백적표체,이자여종류내BCL-2급COX-2단백적표체유현저차이(P<0.001),여직장량성식육방장점막조직중COX-2단백화BCL-2단백적표체적무차이(P>0.05),이행점막내COX-2、BCL-2단백적표체여비이행점막적표체적차별무통계학의의(P>0.05).결론:직장암원단TM내암기인단백COX-2화BCL-2단백적표체무특이성,이행점막병비시암전병변;직장암원단절제2 cm이족구.
Objective:To detect the expressions of COX-2 protein and BCL-2 protein in transitional mucosa adjacent to rectal carcinoma,and determine whether the transitional mucosa was the cancer precursor event.Methods:Mucin histochemical method was employed to detect the distal mucosa 2 cm away from rectal tumor and the transitional mucosa was found in 54 cases of rectal carcinoma.Immunohistochemical method was used to investigate the expressions of BCL-2 and COX-2 protein in the specimen of rectal carcinoma mass,transitional mucosa and non-transitional mucosa,and 20 cases of normal rectal mucosa,and the points of the expressions of COX-2 protein and BCL-2 protein were counted.Results:35.19%(19/54)of distal mucosa were characterized as the transitional mucosa.The expressions of COX-2 and BCL-2 protein were detected in carcinoma mass and distal mucosa.Significant difference was observed in carcinoma mass and transitional mucosa(p<0.01).But there were no significant differences between the expressions of those in transitional mucosa and non-transitional mucosa(p>0.05),as well as transitional mucosa and normal mucosa(p>0.05).Conclusion:The performance of transitional mucosa was not the cancer precursor event,but the non-specificity reaction of carcinoma or inflammation.