中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
6期
495-497
,共3页
龙红梅%王晓%李凤英%聂爱芳%刘赟%吴铃%周丽斌%陈焕珍%张翠平%李果
龍紅梅%王曉%李鳳英%聶愛芳%劉赟%吳鈴%週麗斌%陳煥珍%張翠平%李果
룡홍매%왕효%리봉영%섭애방%류빈%오령%주려빈%진환진%장취평%리과
白藜芦醇%胰岛素分泌%钙离子%脂毒性
白藜蘆醇%胰島素分泌%鈣離子%脂毒性
백려호순%이도소분비%개리자%지독성
Resveratrol%Insulin secretion%Calcium ion%Lipotoxicity
分离SD大鼠胰岛接种于24孔板中,用不同浓度的葡萄糖和白藜芦醇分别培养1 h或24 h,结果表明白藜芦醇孵育大鼠胰岛1 h可呈剂苗依赖地抑制大鼠高糖刺激的胰岛素分泌,1、10和100 μmol/L白藜芦醇可以分别使胰岛素的分泌降低10%、35%(P<0.05)和80%(P<0.01).显微离子成像技术爪10μmol/L的白藜芦醇可以使高糖引起的β细胞内Ca2+浓度的升高减少60%(P<0.05).白藜芦醇可使软脂酸孵育24 h大鼠胰岛的胰岛素分泌恢复到对照组的75%(P<0.01),提示白藜芦醇短期可通过调控细胞内的Ca+浓度,而抑制原代胰岛高精刺激的胰岛素分泌,长期可改善软脂酸引起的β细胞损伤.
分離SD大鼠胰島接種于24孔闆中,用不同濃度的葡萄糖和白藜蘆醇分彆培養1 h或24 h,結果錶明白藜蘆醇孵育大鼠胰島1 h可呈劑苗依賴地抑製大鼠高糖刺激的胰島素分泌,1、10和100 μmol/L白藜蘆醇可以分彆使胰島素的分泌降低10%、35%(P<0.05)和80%(P<0.01).顯微離子成像技術爪10μmol/L的白藜蘆醇可以使高糖引起的β細胞內Ca2+濃度的升高減少60%(P<0.05).白藜蘆醇可使軟脂痠孵育24 h大鼠胰島的胰島素分泌恢複到對照組的75%(P<0.01),提示白藜蘆醇短期可通過調控細胞內的Ca+濃度,而抑製原代胰島高精刺激的胰島素分泌,長期可改善軟脂痠引起的β細胞損傷.
분리SD대서이도접충우24공판중,용불동농도적포도당화백려호순분별배양1 h혹24 h,결과표명백려호순부육대서이도1 h가정제묘의뢰지억제대서고당자격적이도소분비,1、10화100 μmol/L백려호순가이분별사이도소적분비강저10%、35%(P<0.05)화80%(P<0.01).현미리자성상기술조10μmol/L적백려호순가이사고당인기적β세포내Ca2+농도적승고감소60%(P<0.05).백려호순가사연지산부육24 h대서이도적이도소분비회복도대조조적75%(P<0.01),제시백려호순단기가통과조공세포내적Ca+농도,이억제원대이도고정자격적이도소분비,장기가개선연지산인기적β세포손상.
Isolated freshly rat islets were transferred to 24-well plates and incubated with different concentrations of glucose or resveratrol for 1 or 24 h.The results showed that resveratrol dose-dependently inhibited glucose-stimulated insulin secretion from isolated rat islets after 1 h incubation,with 10%,35%,and 80% (P<0.05 or P<0.01) decrease at the concentrations of 1,I0,and 100 μmol/L.10 μmol/L resveratrol decreased the intracellular calcium concentration by 60% (P<0.05).After incubation for 24 h,resveratrol increased palmitatesuppressed insulin secretion to 75% (P<0.01) of control.These results suggest that resveratrol acutely inhibits insulin secretion from primary pancreatic islet via regulating intracellular calcium ion concentration,and in the long run resveratrol may protect β-cells from lipotoxicity.
