CAJ | 학술논문

目的研究外源性视黄酸(RA)对人巩膜成纤维细胞(HSF)生长的调控,以及对基质金属蛋白酶2(MMP-2)和基质金属蛋白酶2组织抑制剂(TIMP-2)mRNA水平的影响.方法体外培养HSF,取第5～7代细胞,经10-10、10-9、10-8、10-7、10-6mol/L浓度的RA作用6 d后,进行细胞计数,观察RA对HSF生长的调控情况;用Real-time PCR检测RA作用后HSF中MMP-2、TIMP-2的mRNA水平.对不同浓度组问比较采用单因素方差分析,两两比较采用t检验.结果 RA作用HSF 6 d后,浓度≥10-9 mol/L RA组对细胞的生长抑制作用差异均有统计学意义(P<0.05),随着RA浓度增高,细胞数量逐渐减少,抑制作用呈剂量效应.RA作用6 d后,RA≥10-8 mol/L时,HSF中MMP-2 mRNA水平有升高趋势,但各组差异无统计学意义(P>0.05);RA≥10-9 mol/L时,TIMP-2 mRNA水平下降(P<0.05).结论 RA能够抑制HSF的生长,并可能通过调控TIMP-2的mRNA水平,使巩膜主动重塑.
목적 연구외원성시황산(RA)대인공막성섬유세포(HSF)생장적조공,이급대기질금속단백매2(MMP-2)화기질금속단백매2조직억제제(TIMP-2)mRNA수평적영향.방법 체외배양HSF,취제5～7대세포,경10-10、10-9、10-8、10-7、10-6mol/L농도적RA작용6 d후,진행세포계수,관찰RA대HSF생장적조공정황;용Real-time PCR검측RA작용후HSF중MMP-2、TIMP-2적mRNA수평.대불동농도조문비교채용단인소방차분석,량량비교채용t검험.결과 RA작용HSF 6 d후,농도≥10-9 mol/L RA조대세포적생장억제작용차이균유통계학의의(P<0.05),수착RA농도증고,세포수량축점감소,억제작용정제량효응.RA작용6 d후,RA≥10-8 mol/L시,HSF중MMP-2 mRNA수평유승고추세,단각조차이무통계학의의(P>0.05);RA≥10-9 mol/L시,TIMP-2 mRNA수평하강(P<0.05).결론 RA능구억제HSF적생장,병가능통과조공TIMP-2적mRNA수평,사공막주동중소.
Objective To study effects of exogenous retinoic acid (RA) on the growth of human scleral fibroblasts (HSF) and the mRNA level of matrix metalloproteinase-2 (MMP-2) and its tissue inhibitor (TIMP-2). Methods HSF of 5-7 passages were quantitated after being treated with RA at concentrations of 10-10 mol/L, 10-9 mol/L,10-8 mol/L, 10-7 mol/L, 10-6 mol/L, respectively for 6 days. The mRNA level of MMP-2 and TIMP-2 was measured by Real-time PCR followd by analysis using one-way ANOVA. Results The growth of HSF was significantly inhibited after 6 days of treatment with at least 10-9 mol/L of RA (P<0.05). The reduction in number of HSF was positively correlated to the RA concentrations used. The mRNA level of MMP-2 was increased when the concentration of RA > 10-8 mol/L. However, there was not significantly different between RA treated and non-RA treated HSF (P>0.05). The mRNA level of TIMP-2 decreased (P<0.05) in HSF treated with RA at concentration ≥10-9 mol/L. Conclusion RA could inhibit the growth of HSF cells and downregulate the mRNA level of TIMP-2, probably resulting in scleral remodeling.