CAJ | 학술논문

目的探讨左旋棉酚对人鼻咽癌细胞株CNE2凋亡的影响及其机制.方法采用MTT实验检测左旋棉酚对CNE2细胞的增殖抑制作用,流式细胞术分析左旋棉酚诱导细胞凋亡及其对Bc1-2、Bax蛋白表达的影响,caspase-3分光光度法检测试剂盒测定caspase-3活性.结果 ≥10μmol/L左旋棉酚可显著抑制CNE2细胞增殖,并表现出剂量、时间依赖效应.左旋棉酚可调节CNE2细胞的细胞周期,使其主要被阻滞于G0/G1期,在诱导细胞凋亡过程中,凋亡相关基因Bcl-2表达下降,而Bax表达则相对上调,caspase-3活性在24 h达到高峰.结论左旋棉酚在体外可诱导鼻咽癌细胞CNE2凋亡,其机制可能与Bcl-2基因下调、Bax基因上调、caspase-3的激活有关.
목적 탐토좌선면분대인비인암세포주CNE2조망적영향급기궤제.방법 채용MTT실험검측좌선면분대CNE2세포적증식억제작용,류식세포술분석좌선면분유도세포조망급기대Bc1-2、Bax단백표체적영향,caspase-3분광광도법검측시제합측정caspase-3활성.결과 ≥10μmol/L좌선면분가현저억제CNE2세포증식,병표현출제량、시간의뢰효응.좌선면분가조절CNE2세포적세포주기,사기주요피조체우G0/G1기,재유도세포조망과정중,조망상관기인Bcl-2표체하강,이Bax표체칙상대상조,caspase-3활성재24 h체도고봉.결론 좌선면분재체외가유도비인암세포CNE2조망,기궤제가능여Bcl-2기인하조、Bax기인상조、caspase-3적격활유관.
Objective To investigate the apoptosis-inducing effect of L-gossypol on human nasopharyngesl carcinoma cell line CNE2 and its possible mechanism.Methods The effect of L-gossypol on proliferation of CNE2 cells was estimated by MTT assay.Flow cytometry was used to analyz cell apoptosis induced by(-)-gossypol and the expression of Bcl-2 、Bax proteins.Caspase-3 activity was determined by caspase-3 colorimetric assay kit.Results Lgossypol was able to inhibit the proliferation of CNE2 cells in a dose-dependent and time-dependent fashion at concentrations more than 10 μmoL/L.L-gossypol regulated cell cycle and GO/G1 arrest could be observed in CNE2 cells.In the process of apoptosis,the expression of Bcl-2 was down-regulated and Bax was up-regulated,caspase-3 activity was in peak at 24h.Conclusion L-gossypol could induce apoptosis in nasopharyngeal carcinoma cell line CNE2 in vitro,which may be associated with down-regulation of Bcl-2,up-regulation of Bax genes expression and caspase-3 activation.