CAJ | 학술논문

目的探讨原因不明习惯性流产(URSA)与细胞免疫功能变化及主动免疫治疗机制的关系.方法采用流式细胞仪测定并比较112例原因不明习惯性流产患者和30例健康已生育妇女及76例经主动免疫治疗后的URSA者外周血CD3+、CD4+、CD8+、CD16+CD56+未细胞亚群比例及CD4+/CD8+的比值;同时比较76例采用主动免疫治疗和36例未采用主动免疫治疗的URSA者再次妊娠成功率.结果 URSA者与健康已生育妇女比较,血中CD3+、CD16+CD56+未细胞的百分率及CD4+/CW4+比值增高,差异有统计学意义(P<0.05).主动免疫后CD3+、CD16+CD56+细胞百分率及CD4+/CD8+比值下降(P<0.05).经主动免疫治疗的URSA者再次妊娠成功率为88.2%,对照组为31.2%,两组比较差异有统计学意义(P<0.01).结论淋巴细胞亚群比例的改变与URSA的发生有关,主动免疫治疗可调节异常的细胞免疫功能,有利于提高再次妊娠成功率.
목적 탐토원인불명습관성유산(URSA)여세포면역공능변화급주동면역치료궤제적관계.방법 채용류식세포의측정병비교112례원인불명습관성유산환자화30례건강이생육부녀급76례경주동면역치료후적URSA자외주혈CD3+、CD4+、CD8+、CD16+CD56+미세포아군비례급CD4+/CD8+적비치;동시비교76례채용주동면역치료화36례미채용주동면역치료적URSA자재차임신성공솔.결과 URSA자여건강이생육부녀비교,혈중CD3+、CD16+CD56+미세포적백분솔급CD4+/CW4+비치증고,차이유통계학의의(P<0.05).주동면역후CD3+、CD16+CD56+세포백분솔급CD4+/CD8+비치하강(P<0.05).경주동면역치료적URSA자재차임신성공솔위88.2%,대조조위31.2%,량조비교차이유통계학의의(P<0.01).결론 림파세포아군비례적개변여URSA적발생유관,주동면역치료가조절이상적세포면역공능,유리우제고재차임신성공솔.
Objective To investigate the relation between the cellular immune function and unexplained recurrent spontaneous abortion(URSA) and the mechanism of active immunotherapy on URSA patients.Methods The flow cytometry(FCM) was used to detect CD3+ ,CD4+ ,CD8+ T lymphocyte and CD16+CD56+ natural killer(NK) cell subsets and the ratio of CD4+/CD8+ of peripheral blood(PBL).112 cases with URSA(76 cases treated with active immunotherapy) and 30 cases of normal fertiled(NF) women were studied.The percentages of T lymphocyte and NK cell subsets before and after therapy were compared among 76 cases wtih URSA treated by active immunotherapy.The rate of next successful pregnancy of URSA patients treated with and without active immunotherapy was compared.Results The percentages of CD3+ and CD16+CD56+ cell subsets as well as the ratio of CD4+/CD8+ of the URSA patients were significantly higher than those of NF cases(P <0.05).After active immunotherapy,the percentages of CD3+ and CD56+CD16+ cell subsets as well as the ratio of CD4+/CD8+ of URSA cases were significantly decreased(P <0.05 ).The rate of next successful pregnancy of URSA cases with and without active immunotherapy were 88.2% and 31.2% respectively,the difference was significant(P< 0.05).Conclusion The changes in the percentages of T lympyocyte and NK cell subsets have something to do with URSA.Active immunotherapy can effectively regulate the cellular immune function and increase the rate of next successful pregnancy of URSA patients.