CAJ | 학술논문

目的探讨细胞周期蛋白A(Cyclin A)在儿童急性白血病患者中的表达及临床意义.方法应用Western blot方法检测47例初诊急性白血病(AL)患儿和33例非血液系统恶性疾病患儿(对照组)骨髓单个核细胞Cyclin A的表达.结果 47例AL患儿Cyclin A表达水平(0.38±0.20)显著高于对照组(0.03±0.15)(P<0.01);急性淋巴细胞白血病(ALL)高危组(HR-ALL)患儿Cyclin A表达水平(0.62±0.38)高于ALL标危组(SR-ALL)(0.33±0.33)(P<0.05);33例外周血WBC≥50 × 109/L患儿组和14例外周血WBC<50×109/L患儿组Cyclin A的表达水平分别为0.64±0.36和0.39±0.38,差异有统计学意义(P<0.05);Cyclin A表达阳性的18例患儿中8例完全缓解(CR),CR率44.4%,较阴性患儿CR率(100%)低(P<0.01).结论 Cyelin A参与儿童急性白血病的发生、发展.
목적 탐토세포주기단백A(Cyclin A)재인동급성백혈병환자중적표체급림상의의.방법 응용Western blot방법검측47례초진급성백혈병(AL)환인화33례비혈액계통악성질병환인(대조조)골수단개핵세포Cyclin A적표체.결과 47례AL환인Cyclin A표체수평(0.38±0.20)현저고우대조조(0.03±0.15)(P<0.01);급성림파세포백혈병(ALL)고위조(HR-ALL)환인Cyclin A표체수평(0.62±0.38)고우ALL표위조(SR-ALL)(0.33±0.33)(P<0.05);33예외주혈WBC≥50 × 109/L환인조화14예외주혈WBC<50×109/L환인조Cyclin A적표체수평분별위0.64±0.36화0.39±0.38,차이유통계학의의(P<0.05);Cyclin A표체양성적18례환인중8례완전완해(CR),CR솔44.4%,교음성환인CR솔(100%)저(P<0.01).결론 Cyelin A삼여인동급성백혈병적발생、발전.
Objective To investigate the expression of cyclin A protein in childhood acute leukemia (AL) and its significance. Methods By using Western blotting analysis, cyclin A protein in bone marrow mononuclear cells from 47 newly diagnosed AL children and 33 non-hematological malignancy children was detected. Results The expression of cyclin A in AL group (0.38±0.20) was higher than that in control group (0.03 ± 0.15) (P < 0.01). The expression of cycliu A in high risk acute lymphocyte leukemia (ALL) group (HR-ALL) (0.62±0.38)was higher than that in standard risk ALL group (SR-ALL) (0.33±0.33) (P<0.05). The expression of cyclin A in WBC≥50 × 109/L group and in WBC <50 × 109/L group was (0.64±0.36) and (0.39±0.38), respectively(P <0.05). Eight (44.4%) out of 18 patients with posi-tive cyclin A expression achieved complete remission(CR). The CR rate was lower than that of patients with negative cyclin A expression (100%) (P < 0.01). Conclusions The higher expression of cycliu A may predict a poor prognosis for childhood ALL.