时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
1期
234-236
,共3页
迷迭香酸%大孔吸附树脂%迷迭香%分离%纯化
迷迭香痠%大孔吸附樹脂%迷迭香%分離%純化
미질향산%대공흡부수지%미질향%분리%순화
Rosmarinic acid%Macroporous absorption resin%Rosmarinus officinalis L.%Separation%Purification
目的 考察大孔吸附树脂对迷迭香酸的吸附性能和纯化效果,寻找从迷迭香中分离纯化迷迭香酸的较优工艺.方法 以迷迭香酸的吸附量、解吸率和所得粉末中迷迭香酸的含量为指标,从4种大孔吸附树脂中筛选出较好的D101树脂.通过静态和动态实验,对迷迭香酸在D101树脂上吸附和解吸的条件进行考察和优化.结果 采用D101树脂从迷迭香中分离纯化迷迭香酸的较优工艺参数为:上柱液pH 3～4,速度2 BV/h,溶液处理量9 BV,洗脱剂为40%乙醇,洗脱速度1 BV/h,收集洗脱液3 BV.按此工艺条件,迷迭香酸的解吸率为94.8%,3 BV洗脱液浓缩干燥后,所得粉末中迷迭香酸含量为23.7%,树脂经8次重复使用,吸附量和分离效果无明显下降,可以重复使用.结论 D101树脂可从迷迭香中较好的分离纯化迷迭香酸,具有潜在的工业应用价值.
目的 攷察大孔吸附樹脂對迷迭香痠的吸附性能和純化效果,尋找從迷迭香中分離純化迷迭香痠的較優工藝.方法 以迷迭香痠的吸附量、解吸率和所得粉末中迷迭香痠的含量為指標,從4種大孔吸附樹脂中篩選齣較好的D101樹脂.通過靜態和動態實驗,對迷迭香痠在D101樹脂上吸附和解吸的條件進行攷察和優化.結果 採用D101樹脂從迷迭香中分離純化迷迭香痠的較優工藝參數為:上柱液pH 3～4,速度2 BV/h,溶液處理量9 BV,洗脫劑為40%乙醇,洗脫速度1 BV/h,收集洗脫液3 BV.按此工藝條件,迷迭香痠的解吸率為94.8%,3 BV洗脫液濃縮榦燥後,所得粉末中迷迭香痠含量為23.7%,樹脂經8次重複使用,吸附量和分離效果無明顯下降,可以重複使用.結論 D101樹脂可從迷迭香中較好的分離純化迷迭香痠,具有潛在的工業應用價值.
목적 고찰대공흡부수지대미질향산적흡부성능화순화효과,심조종미질향중분리순화미질향산적교우공예.방법 이미질향산적흡부량、해흡솔화소득분말중미질향산적함량위지표,종4충대공흡부수지중사선출교호적D101수지.통과정태화동태실험,대미질향산재D101수지상흡부화해흡적조건진행고찰화우화.결과 채용D101수지종미질향중분리순화미질향산적교우공예삼수위:상주액pH 3～4,속도2 BV/h,용액처리량9 BV,세탈제위40%을순,세탈속도1 BV/h,수집세탈액3 BV.안차공예조건,미질향산적해흡솔위94.8%,3 BV세탈액농축간조후,소득분말중미질향산함량위23.7%,수지경8차중복사용,흡부량화분리효과무명현하강,가이중복사용.결론 D101수지가종미질향중교호적분리순화미질향산,구유잠재적공업응용개치.
Objective To evaluate the absorption capability and purification effect of rosmarinic acid on macroporous absorption resin, and to find a better method to separate and purify rosmarinic acid from Rosmarinus officinalis L.. Methods Macroporous resin D101 was more suitable for the separation and purification of rosmarinic acid among four kinds of resins selected with absorption capacity, desorption ratio and the content of rosmarinic acid in the powder obtained as criteria.Optimal conditions for absorption and elution of rosmarinic acid were performed with static and dynamic experiments.Results The result showed that the optimum technology parameters were as follows:the pH value of solution 3 to 4, flow rate 2 BV/h, solution treatment capacity 9 BV, and rosmarinic acid absorbed can be desorbed by 40% alcohol at flow rate 1 BV/h, and collected 3 BV eluent. The desorption ratio of rosmarinic acid was 94.8% according to the absorption and desorption conditions above, and the content of rosmarinic acid in powder obtained from 3 BV eluent through concentration and dry was 23.7%. After eight times of repeated use, the absorption capability and separation effect of the macroporous resin was not decreased obviously, and the macroporous resin could be recycled.Conclusion Rosmarinic acid in Rosmarinus officinalis L. can be effectively separated and purified with the macroporous absorption resin D101, and has the potential application value in industry.
