中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2010年
1期
27-29
,共3页
酮康唑%替硝唑%双波长等吸收
酮康唑%替硝唑%雙波長等吸收
동강서%체초서%쌍파장등흡수
Ketoconazole%Tinidazole%Two wavelength equal-absorbance
目的 建立同时测定酮康唑和替硝唑含量的双波长等吸收紫外分光光度法.方法 建立双波长法测定酮康唑和替硝唑含量的标准曲线,并考察检测方法的精密度和回收率.结果 选择317nm作为替硝唑的测定波长,221nm作为酮康唑的含量测定波长,并以352nm作为参比波长.二者标准曲线方程为:A=0.029C+0.0168;A=0.456C-0.0742,高、中、低浓度的平均回收率分别为(100.2±0.70)%;(99.4±0.92)%,测定的日内精密度RSD分别为0.62%,1.03%;日间精密度RSD为1.13%,1.42%.结论 双波长等吸收紫外光度法同时测定酮康唑和替硝唑含量的方法学特异性好,精密度和回收率符合测定要求,测定结果可靠.
目的 建立同時測定酮康唑和替硝唑含量的雙波長等吸收紫外分光光度法.方法 建立雙波長法測定酮康唑和替硝唑含量的標準麯線,併攷察檢測方法的精密度和迴收率.結果 選擇317nm作為替硝唑的測定波長,221nm作為酮康唑的含量測定波長,併以352nm作為參比波長.二者標準麯線方程為:A=0.029C+0.0168;A=0.456C-0.0742,高、中、低濃度的平均迴收率分彆為(100.2±0.70)%;(99.4±0.92)%,測定的日內精密度RSD分彆為0.62%,1.03%;日間精密度RSD為1.13%,1.42%.結論 雙波長等吸收紫外光度法同時測定酮康唑和替硝唑含量的方法學特異性好,精密度和迴收率符閤測定要求,測定結果可靠.
목적 건립동시측정동강서화체초서함량적쌍파장등흡수자외분광광도법.방법 건립쌍파장법측정동강서화체초서함량적표준곡선,병고찰검측방법적정밀도화회수솔.결과 선택317nm작위체초서적측정파장,221nm작위동강서적함량측정파장,병이352nm작위삼비파장.이자표준곡선방정위:A=0.029C+0.0168;A=0.456C-0.0742,고、중、저농도적평균회수솔분별위(100.2±0.70)%;(99.4±0.92)%,측정적일내정밀도RSD분별위0.62%,1.03%;일간정밀도RSD위1.13%,1.42%.결론 쌍파장등흡수자외광도법동시측정동강서화체초서함량적방법학특이성호,정밀도화회수솔부합측정요구,측정결과가고.
Objective To establish the two-wavelength ultraviolet spectrophotometry (TWUS) on the assay of ketoconazole and tinidazole. Methods Standard curves were established to assay ketoconazole and tinidazole, and the verified items included precision and rate of recovery were also investigated. Results Wavelength of 317nm was chosen to detect the content of tinidazole and wavelength of 221nm and 352nm was chosen as a pair of equal point of absorbance, which was used to detect the content of ketoconazole. The standard equations of the line were A=0.029C+0.0168; A4=0.456C-0.0742. The average relative rates of recovery in three levels were (100.2 ±0.70) % and (99.4 ± 0.92) %. During one day, the RSD of precision were 1.13% and 1.42%, and within days, the RSD of precision were 1.13% and 1.42%. Conclusions All the data of each verified item are in accordance with the national relevant rules, which indicate that two wavelength ultraviolet spectrophotometry is a reliable method for simultaneous determination of ketoconazole and tinidazole.
