CAJ | 학술논문

将亚麻与红麻的愈伤组织在水解酶的作用下分离出原生质体,然后利用PEG法使这2种原生质体融合.结果表明,用2%纤维素酶+l%果胶酶+0.55 mol/L甘露醇处理材料,酶解6 h后,可以获得大量游离的、圆球形的原生质体;利用40%PEG6000及0.3 mol/L CaCI2溶液(含9%甘露醇,pH 9.5)将这2种原生质体融合,可以获得稳定的杂合细胞.
장아마여홍마적유상조직재수해매적작용하분리출원생질체,연후이용PEG법사저2충원생질체융합.결과표명,용2%섬유소매+l%과효매+0.55 mol/L감로순처리재료,매해6 h후,가이획득대량유리적、원구형적원생질체;이용40%PEG6000급0.3 mol/L CaCI2용액(함9%감로순,pH 9.5)장저2충원생질체융합,가이획득은정적잡합세포.
With the method of enzymolysis.the protoplasts were released from the callus of Linum usitatissimum L and Hibiscus cannabius L.,respectively,and then they were fused by PEG as fusogen.The results showed that spherical protoplasts were available in sufficieut quantities by using 2% cellulase+1%peetinase+0.5 mol/L mannitol to treat the callUS for 6 h. Furthermore,the two different protoplasts could be fused into heterozygous pmtoplasts using 40%PEG fusion liquid and 0.3 mol/L CaCl2 solution(containing 9%mannitol,pH value 9.5),and these heterozygous protoplasts were stable.