CAJ | 학술논문

为了明确玉米弯孢叶斑病菌的侵入机理,对农杆菌介导法（ATMT）转化弯孢叶斑病菌的条件进行了探究。以农杆菌AGL-1为侵染菌系、pCAMDGFP作为转化载体。结果表明,玉米弯孢叶斑病菌的ATMT最佳转化条件为：分生孢子萌发12 h后,与农杆菌在25℃黑暗条件下共培养48 h,共培养介质为微孔滤膜。转化效率可达到100～110个转化子/106个孢子,并获得转报道基因GFP的弯孢叶斑病菌单孢。
위료명학옥미만포협반병균적침입궤리,대농간균개도법（ATMT）전화만포협반병균적조건진행료탐구。이농간균AGL-1위침염균계、pCAMDGFP작위전화재체。결과표명,옥미만포협반병균적ATMT최가전화조건위：분생포자맹발12 h후,여농간균재25℃흑암조건하공배양48 h,공배양개질위미공려막。전화효솔가체도100～110개전화자/106개포자,병획득전보도기인GFP적만포협반병균단포。
In order to analyze the Curvularia lunata ingression mechanism,this study explored the main factors affecting Agrobacterium-mediated Curvularia lunata.The recombinant vector pCAMDGFP was transformed in this study into fresh conidia of Curvularia lunata via Agrobacterium tumefaciens（AGL-1）.The results showed that germination time of Curvularia lunata conidia was 12 h at 25 ℃;co-culturing time of the germinated conidia and bacteria was 48 h at 25 ℃;co-culturing media was millipore filter;and the transformation frequency was up to 100-110 transformants per 106 conidia.Transgenic GFP Curvularia lunata spores were also generated in this study.