CAJ | 학술논문

目的了解广东地区急性呼吸道感染患儿人类博卡病毒(HBoV)的感染情况.方法收集广东地区2007年6月至2008年5月期间呼吸道感染患儿的鼻咽分泌物447份,采用PCR法检测HBoV衣壳蛋白(VP)基因片段,阳性标本作核酸序列测定,并与基冈库中的已知序列进行序列比对和系统进化树分析.结果 447例呼吸道感染患儿标本中HBoV阳件率为5.1%.其中10例患儿与其他病毒混合感染,占阳性标本的43.5%.阳件患儿的主要临床诊断为喘息性肺炎、毛细支气管炎和支气管肺炎,年龄分布从42 d到6岁,主要集中在1岁以内,HBoV感染的季节分布偏向夏、秋及晚春.经序列比对和进化树分析.阳性株的VP基因片段与瑞典株ST1的核酸及氨基酸序列同源性分别为97.8%～98.8%及99.3%～100.0%.结论 HBoV是广东地区儿童下呼吸道感染的重要病原之一,且在1岁以内患儿中高发.该地区HBoV流行株的VP基因片段较为保守,但也存在导致氨基酸改变的突变株.
목적 료해엄동지구급성호흡도감염환인인류박잡병독(HBoV)적감염정황.방법 수집엄동지구2007년6월지2008년5월기간호흡도감염환인적비인분비물447빈,채용PCR법검측HBoV의각단백(VP)기인편단,양성표본작핵산서렬측정,병여기강고중적이지서렬진행서렬비대화계통진화수분석.결과 447례호흡도감염환인표본중HBoV양건솔위5.1%.기중10례환인여기타병독혼합감염,점양성표본적43.5%.양건환인적주요림상진단위천식성폐염、모세지기관염화지기관폐염,년령분포종42 d도6세,주요집중재1세이내,HBoV감염적계절분포편향하、추급만춘.경서렬비대화진화수분석.양성주적VP기인편단여서전주ST1적핵산급안기산서렬동원성분별위97.8%～98.8%급99.3%～100.0%.결론 HBoV시엄동지구인동하호흡도감염적중요병원지일,차재1세이내환인중고발.해지구HBoV류행주적VP기인편단교위보수,단야존재도치안기산개변적돌변주.
Objective To investigate the prevalence of human bocavirus(HBoV)among children with acute respiratory tract infection(ARTI)in Guangdong Province.Methods Four hundred and forty-seven nasopharyngeal aspirates or swabs samples from children with ARTI in Guangdong Province were collected from June 2007 to May 2008.HBoV capsid protein VP gene fragments were detected using polymerase chain reaction(PCR).Positive PCR products were sequenced.The DNA and translated amino acid sequences were aligned with known HBoV sequences in GenBank and phylogenetic analysis was also done.Results The positive rate of HBOV was 5.1%of samples from 447 ARTI cases.Ten samples were positive for both HBoV and other respiratory virus,which was 43.5%of positive samples.The main diagnosis for HBoV positive children included wheezing pneumonia,bronchiolitis and bronchial pneumonia.HBoV positive children ranged from 42 days to 6 years old,and most of them were younger than one year.HBOV infection was more common during summer,early autumn and late spring.Through sequence alignment and phylogenetie analysis,the DNA sequences and amino acid sequences of VP gene fragments of isolated HBoV strains showed 97.8%-98.8%and 99.3%-100.0%identity with ST1,respectively.Conclusions HBoV is one of the important pathogens of lower respiratory tract infection in children in Guangdong Province,which is more prevalent in infants younger than one year.Although VP gene fragment of HBoV is conservative in general,there are still some missense mutations.