中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
3期
169-172
,共4页
李永丽%张立玮%丁国瑾%袁丽%朱晓娅%侯淑芸
李永麗%張立瑋%丁國瑾%袁麗%硃曉婭%侯淑蕓
리영려%장립위%정국근%원려%주효아%후숙예
胃肿瘤%食管肿瘤%双原发癌%CDH1%甲基化
胃腫瘤%食管腫瘤%雙原髮癌%CDH1%甲基化
위종류%식관종류%쌍원발암%CDH1%갑기화
Stomach neoplasms%Esophasus neoplas double primary carcinoma%Esophageal squmaous cell carcinoma%CDH1%Methylatlion
目的 研究同一个体食管和胃双原发癌组织中CDH1基因启动子区CpG岛甲基化变化及其临床意义.方法 应用甲基化特异性PCR法,检测18例食管和胃双原发癌患者癌组织及癌旁组织中CDH1基因甲基化的表达.结果 2007年1月至2009年9月河北医科大学第四医院经内镜确诊18例双原发癌患者,食管鳞状细胞癌及癌旁组织CDH1基因甲基化阳性率分别为66.7%和33.3%,二者间差异有统计学意义(χ2=4.167,P=0.031);胃腺癌及癌旁组织CDH1基因甲基化阳性率分别为77.8%和44.4%,二者间差异无统计学意义(χ2=1.786,P=0.180).同一患者的食管癌和胃癌组织CDH1基因甲基化阳性率差异无统计学意义(P=0.500).18例双原发癌中,16例两种癌组织同时出现该基因甲基化一致性改变,一致性变化发生率为88.9%(一致阳性率为66.7%,一致阴性率为22.2%),统计学分析二者呈显著相关性(P=0.005).结论 双原发癌食管癌和胃癌存在较高的CDH1基因甲基化一致性变化,提示二者可能具有相似的发病因素和分子机制.
目的 研究同一箇體食管和胃雙原髮癌組織中CDH1基因啟動子區CpG島甲基化變化及其臨床意義.方法 應用甲基化特異性PCR法,檢測18例食管和胃雙原髮癌患者癌組織及癌徬組織中CDH1基因甲基化的錶達.結果 2007年1月至2009年9月河北醫科大學第四醫院經內鏡確診18例雙原髮癌患者,食管鱗狀細胞癌及癌徬組織CDH1基因甲基化暘性率分彆為66.7%和33.3%,二者間差異有統計學意義(χ2=4.167,P=0.031);胃腺癌及癌徬組織CDH1基因甲基化暘性率分彆為77.8%和44.4%,二者間差異無統計學意義(χ2=1.786,P=0.180).同一患者的食管癌和胃癌組織CDH1基因甲基化暘性率差異無統計學意義(P=0.500).18例雙原髮癌中,16例兩種癌組織同時齣現該基因甲基化一緻性改變,一緻性變化髮生率為88.9%(一緻暘性率為66.7%,一緻陰性率為22.2%),統計學分析二者呈顯著相關性(P=0.005).結論 雙原髮癌食管癌和胃癌存在較高的CDH1基因甲基化一緻性變化,提示二者可能具有相似的髮病因素和分子機製.
목적 연구동일개체식관화위쌍원발암조직중CDH1기인계동자구CpG도갑기화변화급기림상의의.방법 응용갑기화특이성PCR법,검측18례식관화위쌍원발암환자암조직급암방조직중CDH1기인갑기화적표체.결과 2007년1월지2009년9월하북의과대학제사의원경내경학진18례쌍원발암환자,식관린상세포암급암방조직CDH1기인갑기화양성솔분별위66.7%화33.3%,이자간차이유통계학의의(χ2=4.167,P=0.031);위선암급암방조직CDH1기인갑기화양성솔분별위77.8%화44.4%,이자간차이무통계학의의(χ2=1.786,P=0.180).동일환자적식관암화위암조직CDH1기인갑기화양성솔차이무통계학의의(P=0.500).18례쌍원발암중,16례량충암조직동시출현해기인갑기화일치성개변,일치성변화발생솔위88.9%(일치양성솔위66.7%,일치음성솔위22.2%),통계학분석이자정현저상관성(P=0.005).결론 쌍원발암식관암화위암존재교고적CDH1기인갑기화일치성변화,제시이자가능구유상사적발병인소화분자궤제.
Objective To study the changes of CDH1 gene promoter CpG island methylation and its clinical significance in patients with esophagus and stomach double primary carcinoma(ESDC).Methods The expression of CDH1 gene methylation in cancerous tissues and adjacent cancerous tissues in 18 cases of ESDC were detected using methylation-specific PCR method. Results Eighteen patients were endoscopically diagnosed as ESDC between Jan. 2007 and Sep. 2009 in the 4th Hospital Affiliated to Hebei Medical University. The positive methylation of CDH1 gene in tissues of esophageal squamous cell carcinoma (ESCC)and adjacent cancer were 66.7% and 33. 3%, respectively, with significant difference (χ2= 4. 167, P = 0. 031). Whereas the positive methylation of CDH1 gene in tissues of gastric carcinoma (GA) and adjacent cancer were 77.8% and 44.4%, respectively, without statistical difference (χ2=1.786, P= 0. 180). There was no significant difference (P=0. 500) in positive rate of CDH1 gene methylation between ESCC tissues and GA tissues in same individual with ESDC. For 18 patients with ESDC, consistent change of CDH1 methylation in tissues of two kinds of cancers was found in 16 patients with a total agreement of 88.9 % (positive agreement of 66.7 % and negative agreement of 22. 2%). Statistical analysis showed a significant correlation between two groups (P = 0. 005). Conclusion In patients with ESDC, there is a high consistency of CDH1 methylation change, between ESCC and GA,which suggests that two kinds of cancer may have similar risk factors and molecular mechanisms.
