CAJ | 학술논문

目的探讨心肌缺血/再灌注(I/R)损伤及缺血预处理(IP)的保护作用是否受动脉粥样硬化的影响.方法48只健康Wistar大鼠随机分为2大组:正常大鼠组(NC rats)及动脉粥样硬化大鼠组(AS rats).正常大鼠饲以标准实验室饲料,动脉粥样硬化大鼠连续3 d腹腔注射维生素D3后给以致动脉粥样硬化饲料.每大组动物又随机分为2小组:缺血/再灌注损伤组(I/R group)及缺血预处理组(IP group),NC-I/R和AS-I/R组大鼠给予45 min左室前壁缺血及180 min再灌注;NC-IP和AS-IP组大鼠在2次5 min缺血10 min再灌后给予45 min缺血及180 min再灌.检测指标为:心功能、心梗面积、心肌MPO活性、心肌MDA含量、心肌NO含量及iNOS活性.结果I/R使两组大鼠心肌均受到严重损伤,但AS大鼠损伤更重.IP可以明显减轻两组大鼠心肌I/R损伤,且两组大鼠IP的保护作用相似.结论动脉粥样硬化心脏更易受到I/R损伤,但是IP对动脉粥样硬化心脏仍然具有保护作用,且该作用和IP对正常大鼠的保护作用相似.
목적탐토심기결혈/재관주(I/R)손상급결혈예처리(IP)적보호작용시부수동맥죽양경화적영향.방법48지건강Wistar대서수궤분위2대조:정상대서조(NC rats)급동맥죽양경화대서조(AS rats).정상대서사이표준실험실사료,동맥죽양경화대서련속3 d복강주사유생소D3후급이치동맥죽양경화사료.매대조동물우수궤분위2소조:결혈/재관주손상조(I/R group)급결혈예처리조(IP group),NC-I/R화AS-I/R조대서급여45 min좌실전벽결혈급180 min재관주;NC-IP화AS-IP조대서재2차5 min결혈10 min재관후급여45 min결혈급180 min재관.검측지표위:심공능、심경면적、심기MPO활성、심기MDA함량、심기NO함량급iNOS활성.결과I/R사량조대서심기균수도엄중손상,단AS대서손상경중.IP가이명현감경량조대서심기I/R손상,차량조대서IP적보호작용상사.결론동맥죽양경화심장경역수도I/R손상,단시IP대동맥죽양경화심장잉연구유보호작용,차해작용화IP대정상대서적보호작용상사.
Objective To study whether the myocardial ischemia/reperfusion injury and the protective role of ischemic preconditioning (IP) are affected by atherosclerosis. Methods Forty-eight healthy Wistar rats were randomly divided into 2 groups: normal control rats (NC rats) and atherosclerotic rats (AS rats). NC rats were fed with standard laboratory chow, while AS rats were celiac injected for 3 days with vitamine D3 and then fed with an atherogenic diet. Each group was further randomly divided into 2 subgroups: ischemia/reperfusion (I/R) group and ischemic preconditioning (IP) group. NC-I/R and AS-I/R groups were subjected to 45 min regional ischemia followed by 180 min reperfusion; while NC-IP and AS-IP groups were subjected to two cycles of preconditioning, comprising 5 min regional ischemia plus 10 min reperfusion, prior to 45 min ischemia followed by 180 min reperfusion. Cardiac function,infarct size, myocardial MPO activity, MDA content, NO level and iNOS activity were measured. Results I/R caused pronounced myocardial injuries in both groups with, however,AS rats being more severely impaired than did NC rats. IP could significantly relieve the I/R-induced injuries to approximatdy the same extent in both NC rats and AS rats. Conclusion Atherosclerotic heart is more susceptible to I/R injury. However, the protective role of IP is still existed in the atherosclerotic heart to the same extent as in the normal heart.