药物生物技术
藥物生物技術
약물생물기술
PHARMACEUTICAL BIOTECHNOLOGY
2008年
5期
380-387
,共8页
蒋世云%余龙江%申晓林%熊欣%简艳
蔣世雲%餘龍江%申曉林%熊訢%簡豔
장세운%여룡강%신효림%웅흔%간염
辅酶Q10%芳香族氨基酸%生物合成%沼泽红假单胞菌
輔酶Q10%芳香族氨基痠%生物閤成%沼澤紅假單胞菌
보매Q10%방향족안기산%생물합성%소택홍가단포균
CoQ10%Aromatie amino acids%Biosynthesis%Rhodopseudomnas palustris
CoQ10具有呼吸链电子传递者、抗氧化性、调控基因表达等多种生理生化功能.目前不仅用作药物也用作食品添加剂.微生物发酵法是当前生产CoQ10的主要方法.在细菌中,芳香族氨基酸和CoQ10的苯核环都是通过莽草酸途径合成;它们在生物体中各自的合成存在着相互调控作用.本试验通过在培养过程中添加芳香族氨基酸来考察了其对沼泽红假单胞菌J001合成CoQ10的影响.结果表明:当色氨酸添加量≥15 mg/L时对CoQ10的合成具有强列的抑制作用;苯丙氨酸添加量≥50mg/L时对CoQ10的合成具有一定的抑制作用但当添加量≥75mg/L后对CoQ10的合成变为一定的促进作用;酪氨酸添加量为75～175 mg/L时对CoQ10的合成具有一定的促进作用,但当添加量≥200 mg/L后对CoQ10的合成变为一定的抑制作用.当添加苯丙氨酸100 mg/L+酪氨酸150mg/L时,CoQ10含量达最高(25.6±1.3 mg CoQ10/g干细胞),比不添加对照提高52.2%.以上结果说明:该菌株CoQ10的合成受到3种芳香族氨基酸的调控,3-脱氧-D-阿拉伯己酮糖-7-磷酸合成酶是同工酶系统并主要受色氨酸调控,无色氨酸培养基及在培养期间添加适量的苯丙氨酸与酪氨酸对该菌株合成CoQl.有利.
CoQ10具有呼吸鏈電子傳遞者、抗氧化性、調控基因錶達等多種生理生化功能.目前不僅用作藥物也用作食品添加劑.微生物髮酵法是噹前生產CoQ10的主要方法.在細菌中,芳香族氨基痠和CoQ10的苯覈環都是通過莽草痠途徑閤成;它們在生物體中各自的閤成存在著相互調控作用.本試驗通過在培養過程中添加芳香族氨基痠來攷察瞭其對沼澤紅假單胞菌J001閤成CoQ10的影響.結果錶明:噹色氨痠添加量≥15 mg/L時對CoQ10的閤成具有彊列的抑製作用;苯丙氨痠添加量≥50mg/L時對CoQ10的閤成具有一定的抑製作用但噹添加量≥75mg/L後對CoQ10的閤成變為一定的促進作用;酪氨痠添加量為75～175 mg/L時對CoQ10的閤成具有一定的促進作用,但噹添加量≥200 mg/L後對CoQ10的閤成變為一定的抑製作用.噹添加苯丙氨痠100 mg/L+酪氨痠150mg/L時,CoQ10含量達最高(25.6±1.3 mg CoQ10/g榦細胞),比不添加對照提高52.2%.以上結果說明:該菌株CoQ10的閤成受到3種芳香族氨基痠的調控,3-脫氧-D-阿拉伯己酮糖-7-燐痠閤成酶是同工酶繫統併主要受色氨痠調控,無色氨痠培養基及在培養期間添加適量的苯丙氨痠與酪氨痠對該菌株閤成CoQl.有利.
CoQ10구유호흡련전자전체자、항양화성、조공기인표체등다충생리생화공능.목전불부용작약물야용작식품첨가제.미생물발효법시당전생산CoQ10적주요방법.재세균중,방향족안기산화CoQ10적분핵배도시통과망초산도경합성;타문재생물체중각자적합성존재착상호조공작용.본시험통과재배양과정중첨가방향족안기산래고찰료기대소택홍가단포균J001합성CoQ10적영향.결과표명:당색안산첨가량≥15 mg/L시대CoQ10적합성구유강렬적억제작용;분병안산첨가량≥50mg/L시대CoQ10적합성구유일정적억제작용단당첨가량≥75mg/L후대CoQ10적합성변위일정적촉진작용;락안산첨가량위75～175 mg/L시대CoQ10적합성구유일정적촉진작용,단당첨가량≥200 mg/L후대CoQ10적합성변위일정적억제작용.당첨가분병안산100 mg/L+락안산150mg/L시,CoQ10함량체최고(25.6±1.3 mg CoQ10/g간세포),비불첨가대조제고52.2%.이상결과설명:해균주CoQ10적합성수도3충방향족안기산적조공,3-탈양-D-아랍백기동당-7-린산합성매시동공매계통병주요수색안산조공,무색안산배양기급재배양기간첨가괄량적분병안산여락안산대해균주합성CoQl.유리.
CoQ10 has been used not only as a drug but also as a food supplement because of its various Dhysi ological and biochemical activities.Microbial fermentation is a main way for the commercial production of CoQ10.The quinoid nucleus of CoQ10 and aromatic amino acids are synthesized from the shikimate pathway in bacteria and there are some effects of them on another biosynthesis.The impacts of aromatic amino acids on the biosynthesis of CoQ10 in Rhodopseudomnas palustris J001 were investigated with feeding aromatic amino acids at the end of the logarithmic phase during incubation.The results showed that the feeding of tryptophan(above 15 mg/L) strongly repressed the biosynthesis of CoQ10.There were also some slightly unfavorable effects of feeding of phenylalanine(50 mg/L)and tyrosine(above 200 rag/L)on the biosynthesis of CoQl0.However,the biosynthe sis of CoQ10 could be enhanced by feeding phenylalanine(above 75 mg/L)Or tyrosine(from 75 to 1 75 mg/L).The content of CoQ10 in the strain was found to be the highest up tO(25.6±1.3)mg CoQ10/g dried biomass when feeding phenylalanine(1 00 mg/L)+tyrosine(1 50 mg/L),which was 52.2% higher than that in the control with no additional amino acids in the medium.The results indicated that the biosynthesis of CoqIo was affected by the three aromatic amino acids.It can be concluded that the biosynthesis of CoQ10 in strain is favorable by feeding appropriate amount of phenylalanien and tyrosine without tryptophan.
