农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
6期
39-42
,共4页
孟祥伟%额尔登其木格%红海%李蓓%芒来
孟祥偉%額爾登其木格%紅海%李蓓%芒來
맹상위%액이등기목격%홍해%리배%망래
IGF-Ⅰ%3′Flank region%PCR-SSCP
[Objective] To explore the polymorphism of the 3' flank region of equine IGF-Ⅰ gene. [Method] The 3' flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mongolian horse, Sanhe horse and Thoroughbred, and then analyzed by PCR-SSCP. [Result] Three genotypes (AA, BB and AB) were detected by PCR-SSCP and the distribution of genotypes of all research objects except Xinihe horse and Baerhu horse were in line with the "Hardy-Weinberg Law". [Conclusion] There was a polymorphic locus in the 3' flank region of IGF-Ⅰ gene, which might affect the equine growth and development mechanism. The study is of important theoretical and practical significance to improve the performance and to develop equine industry.
