CAJ | 학술논문

采用5种不同极性的树脂(AB-8、S-8、NKA-9、D-101和X-5)来评价对苜蓿皂苷的吸附和解吸附作用,其中中等极性的AB-8树脂对苜蓿皂苷具有最大的吸附量,用55%～65%的乙醇溶液能有效地将吸附的皂苷洗脱下来.当苜蓿粗提物量和AB-8树脂量为1∶1时,树脂的吸附量达到饱和.采用AB-8树脂,用90%乙醇洗脱,苜蓿提取物的最大解吸附量为108.4 mg/g干重树脂.通过大孔树脂吸附和解吸附,将90%乙醇洗脱液浓缩,皂苷含量(53%)是苜蓿粗提物含量(5.68%)的9倍.结果表明,AB-8大孔吸附树脂可用于苜蓿皂苷的大规模制备.
채용5충불동겁성적수지(AB-8、S-8、NKA-9、D-101화X-5)래평개대목숙조감적흡부화해흡부작용,기중중등겁성적AB-8수지대목숙조감구유최대적흡부량,용55%～65%적을순용액능유효지장흡부적조감세탈하래.당목숙조제물량화AB-8수지량위1∶1시,수지적흡부량체도포화.채용AB-8수지,용90%을순세탈,목숙제취물적최대해흡부량위108.4 mg/g간중수지.통과대공수지흡부화해흡부,장90%을순세탈액농축,조감함량(53%)시목숙조제물함량(5.68%)적9배.결과표명,AB-8대공흡부수지가용우목숙조감적대규모제비.
Five types ofmacmporous adsorption resins namely AB-8,S-8,NKA-9,D-101 and X-5 with Varying polarity were evaluated for the adsorption and desorption of alfalfa saponins.Of these,the moderately polar AB-8 resin showed the highest adsorption capacity and the absorbed saponius could be duted effectively with 55-65% aqueous ethanol.The adtract was 108.4 mg/g dw AB-8 resin on a column eluted by 90% aqneous ethanol.The saponin content in the concentrated 90% aqueous ethanol effluent reached 53%,about 9 times of that(5.68%)in the crude alfalfa extract.The AB8 macroporous resin has may be a favorable sorbent for large-scale saponin isolation from alfalfa.