CAJ | 학술논문

目的试验证实我国黑热病不同疫区分离出的5个L.d分离株的小亚基核糖体DNA可变区序列存在点突变.方法将7个利什曼原虫种/株的nDNA进行PCR扩增，将扩增出的SSU rDNA基因的特异片段克隆于pGEMR-T Easy Vector上，采用通用引物M13进行PCR扩增，全自动测序仪测序。结果序列分析显示，扩增的7种/株利什曼原虫SSU rDNA序列大小为392 bp，5个点突变均发生在两个独特序列区（UQ-I和UQ-II）；无移码突变。经与基因库中利什曼原虫序列类似性比较，同源性在98%以上。结论首次报道我国黑热病不同疫区的5个L.d.分离株的SSU rDNA可变区存在5个点突变。
목적 시험증실아국흑열병불동역구분리출적5개L.d분리주적소아기핵당체DNA가변구서렬존재점돌변.방법장7개리십만원충충/주적nDNA진행PCR확증，장확증출적SSU rDNA기인적특이편단극륭우pGEMR-T Easy Vector상，채용통용인물M13진행PCR확증，전자동측서의측서。결과서렬분석현시，확증적7충/주리십만원충SSU rDNA서렬대소위392 bp，5개점돌변균발생재량개독특서렬구（UQ-I화UQ-II）；무이마돌변。경여기인고중리십만원충서렬유사성비교，동원성재98%이상。결론수차보도아국흑열병불동역구적5개L.d.분리주적SSU rDNA가변구존재5개점돌변。
Objective To confirm the existence of point mutations I n the SSU rDNA variable regions of 5 Leishmania donovani (L-d) isolates from different epidemic foci in ChinaMethods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEMR-T Easy Vectors After that, the specific fragments were sequenced by an auto mated DNA sequencerResults Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 po int mutations were located in two unique sequence blocks (UQ-Ⅰ and UQ-Ⅱ), a nd no insertions or deletions were found The dentities of comparison of Leish mania in GeneBank were more than 98%.Conclusion Five point mutations exist in the ssu rDNA variable region of 5 L.d.isolates from different epidemic foci of visceral leishmaniasis (VL) in China. Sequence differences differences of the SSU rDNA variable region exist among L.d.isolates from different foci.