中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2008年
6期
361-364
,共4页
贾小兵%李露斯%叶建宁%张旭
賈小兵%李露斯%葉建寧%張旭
가소병%리로사%협건저%장욱
昏迷%大鼠%模型%Orexin-A%促醒
昏迷%大鼠%模型%Orexin-A%促醒
혼미%대서%모형%Orexin-A%촉성
coma%rat%model%Orexjn-A%arousal
目的 建立一种新的大鼠缺血性脑损伤昏迷模型,并观察Orexin-A对昏迷大鼠的促醒作用.方法 通过改良后的四血管阻塞法[4-VO+6号(直径0.60 mm)针头"针控线拴法"]建立大鼠缺血性脑损伤昏迷模型.大鼠在昏迷后120 min经侧脑室注射Orexin-A及其受体拮抗剂(SB-334867),观察各组大鼠昏迷时间和脑电、皮质神经元单位放电频率的变化.结果 改良后的4-VO能够明显延长大鼠的昏迷时间,达到6～8 h且动物无死亡,与4-VO+4.5号(直径0.45 mm)和7号(直径0.70 mm)针头"针控线拴法"在昏迷时间上比较差异有统计学意义(F＝344.43,P<O.01).高剂量Orexin-A(4 nmol/10 靗)治疗组大鼠的昏迷时间明显缩短,脑电洳ū壤跎?前额叶皮质神经元单位放电频率增强,与改良后的4-VO组比较差异均有统计学意义(P<0.05或P<0.01);而低剂量Orexin-A(2 nmol/10 靗)治疗组变化不明显.结论 ①改良后的4-VO适合做大鼠缺血性脑损伤昏迷模型.②高剂量的Orexin-A对缺血性脑损伤昏迷大鼠有明显的促醒作用.
目的 建立一種新的大鼠缺血性腦損傷昏迷模型,併觀察Orexin-A對昏迷大鼠的促醒作用.方法 通過改良後的四血管阻塞法[4-VO+6號(直徑0.60 mm)針頭"針控線拴法"]建立大鼠缺血性腦損傷昏迷模型.大鼠在昏迷後120 min經側腦室註射Orexin-A及其受體拮抗劑(SB-334867),觀察各組大鼠昏迷時間和腦電、皮質神經元單位放電頻率的變化.結果 改良後的4-VO能夠明顯延長大鼠的昏迷時間,達到6～8 h且動物無死亡,與4-VO+4.5號(直徑0.45 mm)和7號(直徑0.70 mm)針頭"針控線拴法"在昏迷時間上比較差異有統計學意義(F＝344.43,P<O.01).高劑量Orexin-A(4 nmol/10 靗)治療組大鼠的昏迷時間明顯縮短,腦電洳ū壤跎?前額葉皮質神經元單位放電頻率增彊,與改良後的4-VO組比較差異均有統計學意義(P<0.05或P<0.01);而低劑量Orexin-A(2 nmol/10 靗)治療組變化不明顯.結論 ①改良後的4-VO適閤做大鼠缺血性腦損傷昏迷模型.②高劑量的Orexin-A對缺血性腦損傷昏迷大鼠有明顯的促醒作用.
목적 건립일충신적대서결혈성뇌손상혼미모형,병관찰Orexin-A대혼미대서적촉성작용.방법 통과개량후적사혈관조새법[4-VO+6호(직경0.60 mm)침두"침공선전법"]건립대서결혈성뇌손상혼미모형.대서재혼미후120 min경측뇌실주사Orexin-A급기수체길항제(SB-334867),관찰각조대서혼미시간화뇌전、피질신경원단위방전빈솔적변화.결과 개량후적4-VO능구명현연장대서적혼미시간,체도6～8 h차동물무사망,여4-VO+4.5호(직경0.45 mm)화7호(직경0.70 mm)침두"침공선전법"재혼미시간상비교차이유통계학의의(F＝344.43,P<O.01).고제량Orexin-A(4 nmol/10 정)치료조대서적혼미시간명현축단,뇌전여ū양타?전액협피질신경원단위방전빈솔증강,여개량후적4-VO조비교차이균유통계학의의(P<0.05혹P<0.01);이저제량Orexin-A(2 nmol/10 정)치료조변화불명현.결론 ①개량후적4-VO괄합주대서결혈성뇌손상혼미모형.②고제량적Orexin-A대결혈성뇌손상혼미대서유명현적촉성작용.
Objective To reproduce an ischemic brain injury coma model and explore the arousal effect of Orexin-A.Methods An ischemic brain injury coma model was reproduced in rats by partial four-vessel occlusion(4-VO with a needle of 0.60 mm in diameter in the lumen to create stenosis of the internal carotid arteries).One hundred and twenty minutes after the onset of coma.Orexin-A or its antagonist(SB-334867) was given intraventricularly,and the time of coma and changes in electroencephalogram (ECG) were observed,and the unit discharge of neurons in the prefrontal cortex was recorded.Results Partial occlusion of four internal carotid arteries, reducing the lumens to 0.60 mm, could prolong the time of coma to 6-8 hours with the rats still alive.The duration of coma showed a significant difference compared with that in rats who underwent 0.45 mm or 0.70 mm stenosis of the internal carotid arteries(F=344.43,P<0.01).Intraventricular Orexin-A in a dose of 4 nmol/10 μl could obviously decrease the duration of coma with a decrease in δ wave and increase in unit discharge rate of neurons in coma rats(P<0.05 or P<0.01),but no significant change was observed when the dose was 2 nmol/1O μl.Conclusion ①Creating stenosis of all four internal carotid arteries is suitable to reproduce ischemic brain injury with corna in rats.②Intracere-broventricular injection of Orexin-A (4 nmol) has a potent arousal effect on ischemic brain iniury coma in rats.
