CAJ | 학술논문

目的观察转移性不同的3种膀胱癌细胞株中Raf激酶抑制蛋白(RKIP)的表达,探讨其与膀胱癌侵袭、转移的关系.方法选取膀胱移行细胞癌细胞株EJ、T24和BIU-87,用Western blot检测各细胞株中RKIP的蛋白表达,用逆转录-聚合酶链反应(RT-PCR)检测各系RKIP的mRNA水平,比较它们的差异.结果 EJ、124和BIU-87三者RKIP蛋白相对表达量分别为1.99±0.24、0.82±0.16、0.15±0.03(P<0.05),mRNA相对表达量分别为1.87±0.33、0.86±0.25、0.29±0.12(P<0.05),均依次降低.结论 RKIP可抑制膀胱癌细胞株的侵袭、转移,其表达下调可能在膀胱癌的进展中具有重要作用,RKIP可能与膀胱癌的侵袭和转移呈负相关.
목적 관찰전이성불동적3충방광암세포주중Raf격매억제단백(RKIP)적표체,탐토기여방광암침습、전이적관계.방법 선취방광이행세포암세포주EJ、T24화BIU-87,용Western blot검측각세포주중RKIP적단백표체,용역전록-취합매련반응(RT-PCR)검측각계RKIP적mRNA수평,비교타문적차이.결과 EJ、124화BIU-87삼자RKIP단백상대표체량분별위1.99±0.24、0.82±0.16、0.15±0.03(P<0.05),mRNA상대표체량분별위1.87±0.33、0.86±0.25、0.29±0.12(P<0.05),균의차강저.결론 RKIP가억제방광암세포주적침습、전이,기표체하조가능재방광암적진전중구유중요작용,RKIP가능여방광암적침습화전이정부상관.
Objective To evaluate the relationship between Raf kinase inhibitor protein(RKIP) and bladder cancer metastasis.Methods Thlee kinds of bladder cancer cell lines with different metastatic potentials (transitional cell carcinoma cell lines EJ,T24 and BIU-87)were observed.RKIP protein and mRNA expression was detected with Western blot and RT-PCR,respectively.Differences between the protein and mRNA expressions in each cell line were compared.Results Under normal culture condition,the relative expression of RKIP protein in each cell line(EJ,T24 and BIU-87)was 1.99±0.24,0.82±0.16,and 0.15±0.03(P<0.05),and that of RKIP mRNA was 1.87±0.33,0.86±0.25,and 0.29±0.12 respectively.They were reduced in sequence and there Was significant difference among the three groups(P<0.05).Conclusion RKIP may inhibit the metastasis and invasion of bladder cancer,and its down-regulation may play an important role in bladder cancer progression.