CAJ | 학술논문

目的探讨骨髓增生异常综合征(MDS)患者骨髓克隆细胞表面胰岛素样生长因子Ⅰ型受体(IGF-Ⅰ R)的表达状况.方法联合应用DNA荧光原位杂交(FISH)、免疫细胞化学(碱性磷酸酶-抗碱性磷酸酶系统,APAAP)技术检测40例MDS患者骨髓克隆细胞的IGF-Ⅰ R表达.结果 MDS患者骨髓克隆细胞表面IGF-Ⅰ R表达明显高于正常细胞[分别为(84.5±14.2)％和(11.4±12.1)％](P＜0.01).MDS患者骨髓克隆细胞阳性率与有核细胞IGF-Ⅰ R表达率明显相关(r=0.929,P＜0.01).高危组MDS患者骨髓克隆细胞IGF-Ⅰ R表达与低危组差异无统计学意义.不同异常核型的克隆细胞IGF-Ⅰ R表达率差异也无统计学意义.结论 IGF-Ⅰ R表达阳性提示细胞的恶性增殖倾向,可能作为MDS骨髓恶性克隆细胞的标志物.
목적 탐토골수증생이상종합정(MDS)환자골수극륭세포표면이도소양생장인자Ⅰ형수체(IGF-Ⅰ R)적표체상황.방법 연합응용DNA형광원위잡교(FISH)、면역세포화학(감성린산매-항감성린산매계통,APAAP)기술검측40례MDS환자골수극륭세포적IGF-Ⅰ R표체.결과 MDS환자골수극륭세포표면IGF-Ⅰ R표체명현고우정상세포[분별위(84.5±14.2)％화(11.4±12.1)％](P＜0.01).MDS환자골수극륭세포양성솔여유핵세포IGF-Ⅰ R표체솔명현상관(r=0.929,P＜0.01).고위조MDS환자골수극륭세포IGF-Ⅰ R표체여저위조차이무통계학의의.불동이상핵형적극륭세포IGF-Ⅰ R표체솔차이야무통계학의의.결론 IGF-Ⅰ R표체양성제시세포적악성증식경향,가능작위MDS골수악성극륭세포적표지물.
Objective To probe the expression level of insulin-like growth factor- Ⅰ receptor( IGF-Ⅰ R) in malignant clone cells of myelodysplastic syndromes (MDS).Method The combination of fluorescence in situ hybridization (FISH) and immunochemistry ( alkaline phosphatase anti-alkaline phosphatase,APAAP) was used to detect the expression of IGF- Ⅰ R in the bone marrow cells of 40 MDS patients with abnormal karyotypes.Results The average IGF- Ⅰ R expression level on the clone cells[ (84.5 ± 14.2)％ ]from the MDS cases was markedly elevated compared to the corresponding level on normal cells [ ( 11.4 ±12.1 )％ ].And the percentages of malignant clone cells in all 40 MDS cases were significantly correlated with the relevant percentages of IGF- Ⅰ R positive nucleated cells ( r =0.929,P ＜ 0.01 ).Conclusions IGF- Ⅰ R might be taken as a marker of clone cells in MDS for its trait to malignant proliferation.