肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
2期
76-79
,共4页
陈芾珩%刘元生%苏永忠%陈惜隧%姜美銮%李回军%谭凤娇%侯展文
陳芾珩%劉元生%囌永忠%陳惜隧%薑美鑾%李迴軍%譚鳳嬌%侯展文
진비형%류원생%소영충%진석수%강미란%리회군%담봉교%후전문
血小板生成素%多柔比星%药物毒性%大鼠
血小闆生成素%多柔比星%藥物毒性%大鼠
혈소판생성소%다유비성%약물독성%대서
Thrombopoietin%Adriamycin%Drug toxicity%Rat
目的 通过建立大鼠多柔比星(ADM)急性心肌损伤模型,观察血小板生成素(TPO)对使用多柔比星后大鼠心肌的保护效应.方法 32只Wistar大鼠被随机分成4组,分别为对照组、ADM组、ADM+TPO低剂量(TPOL)组、ADM+TPO高剂量(TPOH)组.对照组给予生理盐水,其余各组给予ADM20mg/kg腹腔内注射,TPO干预组则加用不同剂量的TPO(10 μg/kg或30 μg/kg,隔天1次,共3次).采用ELISA法检测大鼠血清肌酸激酶同工酶(CK-MB)及心肌癌蛋白Ⅰ(cTNI);通过HE染色,观察心肌组织学改变,并利用组织学评分评价心肌损伤程度;电子显微镜下观察心肌细胞超微结构的变化.结果 TPO低、高剂量干预组CK-MB活力分别为(14.65±1.91)ng/ml、(14.21±1.70)ng/ml,cTNI的活力分别为(9.66±1.31)ng/ml、(10.07±1.20)ng/ml,均较ADM组的对应值(19.58±3.49)ng/ml、(12.50±1.62)ng/ml明显下降,P值均<0.05;电子显微镜下ADM组心肌细胞超微结构损害明显,给予TPO后这样的变化减轻;ADM组心肌病理损伤积分较TPO组高(P<0.01);上述指标在ADM+TPOL组及ADM+TPOH组间差异无统计学意义(P>0.05).结论 TPO对大鼠ADM应用后有心肌保护作用.
目的 通過建立大鼠多柔比星(ADM)急性心肌損傷模型,觀察血小闆生成素(TPO)對使用多柔比星後大鼠心肌的保護效應.方法 32隻Wistar大鼠被隨機分成4組,分彆為對照組、ADM組、ADM+TPO低劑量(TPOL)組、ADM+TPO高劑量(TPOH)組.對照組給予生理鹽水,其餘各組給予ADM20mg/kg腹腔內註射,TPO榦預組則加用不同劑量的TPO(10 μg/kg或30 μg/kg,隔天1次,共3次).採用ELISA法檢測大鼠血清肌痠激酶同工酶(CK-MB)及心肌癌蛋白Ⅰ(cTNI);通過HE染色,觀察心肌組織學改變,併利用組織學評分評價心肌損傷程度;電子顯微鏡下觀察心肌細胞超微結構的變化.結果 TPO低、高劑量榦預組CK-MB活力分彆為(14.65±1.91)ng/ml、(14.21±1.70)ng/ml,cTNI的活力分彆為(9.66±1.31)ng/ml、(10.07±1.20)ng/ml,均較ADM組的對應值(19.58±3.49)ng/ml、(12.50±1.62)ng/ml明顯下降,P值均<0.05;電子顯微鏡下ADM組心肌細胞超微結構損害明顯,給予TPO後這樣的變化減輕;ADM組心肌病理損傷積分較TPO組高(P<0.01);上述指標在ADM+TPOL組及ADM+TPOH組間差異無統計學意義(P>0.05).結論 TPO對大鼠ADM應用後有心肌保護作用.
목적 통과건립대서다유비성(ADM)급성심기손상모형,관찰혈소판생성소(TPO)대사용다유비성후대서심기적보호효응.방법 32지Wistar대서피수궤분성4조,분별위대조조、ADM조、ADM+TPO저제량(TPOL)조、ADM+TPO고제량(TPOH)조.대조조급여생리염수,기여각조급여ADM20mg/kg복강내주사,TPO간예조칙가용불동제량적TPO(10 μg/kg혹30 μg/kg,격천1차,공3차).채용ELISA법검측대서혈청기산격매동공매(CK-MB)급심기암단백Ⅰ(cTNI);통과HE염색,관찰심기조직학개변,병이용조직학평분평개심기손상정도;전자현미경하관찰심기세포초미결구적변화.결과 TPO저、고제량간예조CK-MB활력분별위(14.65±1.91)ng/ml、(14.21±1.70)ng/ml,cTNI적활력분별위(9.66±1.31)ng/ml、(10.07±1.20)ng/ml,균교ADM조적대응치(19.58±3.49)ng/ml、(12.50±1.62)ng/ml명현하강,P치균<0.05;전자현미경하ADM조심기세포초미결구손해명현,급여TPO후저양적변화감경;ADM조심기병리손상적분교TPO조고(P<0.01);상술지표재ADM+TPOL조급ADM+TPOH조간차이무통계학의의(P>0.05).결론 TPO대대서ADM응용후유심기보호작용.
Objective To observe the protection effect of thrombopoietin on adriamycin-inducod acute myocardium injury in rats model.Methods 32 Wistar rats were randomized into four groups (n=8):Control group,ADM group,ADM +TPOL group and ADM +TPOH group.All agents were given by intraperitoneal injection.The control group was given normal saline,while the other three groups were given adriamycin at the dosage of 20 mg/kg once.TPO groups were injected TPO at different dosages of 10 μg/kg or 30 μg/kg three times on alternate days.ELISA was used to detect CK-MB and cTnI content of serum in the rats.By HE staining,pathological change was found and grade of tissue morphology was scored.The ultrastructure change of cadiocyte was observed by the electron microscope.Results The energy of CK-MB (14.65±1.91,14.21±1.70) and cTNI (9.66±1.31,10.07±1.20) in TPO groups were obviously lower than that of the ADM group(19.58±3.49,12.50±1.62) (P<0.05),respectively.Pathological score of ADM group was higher than TPO groups (P<0.01).The ultrastructure of myecard tissue in the ADM group was damaged more severely than TPO groups.Above-mentioned indexes were with no significant difference between ADM+TPOL group and ADM+TPOH group.Conclusion TPO can protect the heart which was injured by the ADM.
