CAJ | 학술논문

研究了复苏被子植物牛耳草(Boea hygrometrica (Bunge) R.Br.)离体叶片在微弱光强下(3 μmol photons*m-2*s-1)和黑暗中叶黄素循环组分及叶绿素荧光随脱水复水的变化.结果发现:脱水期间随着光系统Ⅱ光化学效率(Fv/Fm)、实际量子产率(ΦPSⅡ)、光化学淬灭(qP)和非光化学淬灭(NPQ)值的降低,微弱光强下的对照叶片玉米黄素含量显著增加,而微弱光强下DTT处理的叶片和黑暗中的叶片都没有玉米黄素的积累.经过3 d复水后,微弱光强下对照叶片的Fv/Fm, ΦPSⅡ, qP 和 NPQ值能完全恢复,但是微弱光强下DTT处理的叶片和黑暗中的叶片其Fv/Fm、ΦPSⅡ、qP 和 NPQ值只有部分恢复.说明脱水的牛耳草离体叶片光系统Ⅱ的光化学活性的恢复明显受到DTT处理和黑暗的影响,因此玉米黄素可能对微弱光强下脱水的牛耳草叶片具有重要的保护作用.
연구료복소피자식물우이초(Boea hygrometrica (Bunge) R.Br.)리체협편재미약광강하(3 μmol photons*m-2*s-1)화흑암중협황소순배조분급협록소형광수탈수복수적변화.결과발현:탈수기간수착광계통Ⅱ광화학효솔(Fv/Fm)、실제양자산솔(ΦPSⅡ)、광화학쉬멸(qP)화비광화학쉬멸(NPQ)치적강저,미약광강하적대조협편옥미황소함량현저증가,이미약광강하DTT처리적협편화흑암중적협편도몰유옥미황소적적루.경과3 d복수후,미약광강하대조협편적Fv/Fm, ΦPSⅡ, qP 화 NPQ치능완전회복,단시미약광강하DTT처리적협편화흑암중적협편기Fv/Fm、ΦPSⅡ、qP 화 NPQ치지유부분회복.설명탈수적우이초리체협편광계통Ⅱ적광화학활성적회복명현수도DTT처리화흑암적영향,인차옥미황소가능대미약광강하탈수적우이초협편구유중요적보호작용.
The protective role of xanthophyll cycle in resurrection angiosperm Boea hygrometrica (Bunge) R.Br. Was investigated by analysis of the changes of chlorophyll fluorescence and xanthophyll cycle components in response to dehydration and rehydration in detached leaves under very weak light condition (3 μmol photons*m-2*s-1) and in the dark. With declines in the values of PSⅡ photochemical efficiency (Fv/Fm), PSⅡ actual quantum yield (ΦPSⅡ), photochemical quenching (qP) and non-photochemical quenching (NPQ) during dehydration, zeaxanthin significantly increased in control Boea leaves under very weak light condition, while no zeaxanthin accumulation was detected in Boea leaves treated with dithiothreitol (DTT) and Boea leaves in the dark, and after 3 d rehydration, the parameters Fv/Fm, ΦPSⅡ, qP and NPQ showed full recovery in control Boea leaves under very weak light condition, but the parameters only underwent partial recovery in Boea leaves treated with DTT and Boea leaves in the dark, suggesting that the recovery of photosystemⅡ (PSⅡ) photochemical activities in Boea leaves was obviously affected by treatments with DTT and darkness, therefore, zeaxanthin may play an important protective role in desiccated Boea leaves even under very weak light conditions.