CAJ | 학술논문

本试验采用122树脂在不同条件下进行赤霉菌固体发酵产物脱色试验,其中固体培养基提取液用75%、95%和纯水在不同的静态和动态洗脱时间下进行.试验表明酒精提取的赤霉素(GA3)比纯水高10%, 随着提取时间的增加,流出洗脱液中GA3浓度增加,但溶解的色素也在脱色柱中增加.综合各方面,用75%-95%的酒精在122树脂柱中静态洗脱1-3h或振荡洗脱30-60 min最佳.在静态洗脱中,洗脱液几乎无色,透明度0-8.2,GA3浓度834.4-857.3 mg·Kg-1;而在动态洗脱中,洗脱液几乎无色,透明度03.7, GA3浓度865.8-874.7.表3参5.
본시험채용122수지재불동조건하진행적매균고체발효산물탈색시험,기중고체배양기제취액용75%、95%화순수재불동적정태화동태세탈시간하진행.시험표명주정제취적적매소(GA3)비순수고10%, 수착제취시간적증가,류출세탈액중GA3농도증가,단용해적색소야재탈색주중증가.종합각방면,용75%-95%적주정재122수지주중정태세탈1-3h혹진탕세탈30-60 min최가.재정태세탈중,세탈액궤호무색,투명도0-8.2,GA3농도834.4-857.3 mg·Kg-1;이재동태세탈중,세탈액궤호무색,투명도03.7, GA3농도865.8-874.7.표3삼5.
The extracting liquid of gibberellins (Gibberella fujikuroi) from solid medium for was decolorized separately with 75%, 95% alcohol, and distilled water in static adsorption and vibrating way for different durations. The results showed that the content of GA3 in efflux extracted with alcohol is 10% higher than that with distilled water. With the increase of the durations of extraction, the content of GA3 increases and the dissolution of pigments also increase. For extraction of GA3 cultured in solid medium, the best decolorizing result was obtained when it was extracted with 75%-95% alcohol in static way for 1-3 h, or in a vibrating way for 30-60 min, and then to decolor in a static way for 30-60 min in 122 resin column.