CAJ | 학술논문

利用GUS报告基因,建立了农杆菌介导的谷子(Setaria italica)遗传转化体系,并研究了多种影响因素对转化效率的影响,包括受体基因型、外植体类型、菌液浓度、培养基中乙酰丁香酮的浓度、侵染时间和共培养时间.确定了最优的转化条件为:以谷子幼穗诱导的愈伤组织为外植体,用低浓度的农杆菌菌液侵染30～40 min,然后在含有0.1 mmol/L乙酰丁香酮的LS培养基上共培养2 d.
이용GUS보고기인,건립료농간균개도적곡자(Setaria italica)유전전화체계,병연구료다충영향인소대전화효솔적영향,포괄수체기인형、외식체류형、균액농도、배양기중을선정향동적농도、침염시간화공배양시간.학정료최우적전화조건위:이곡자유수유도적유상조직위외식체,용저농도적농간균균액침염30～40 min,연후재함유0.1 mmol/L을선정향동적LS배양기상공배양2 d.
An efficient and repeatable approach in transforming the foxtail millet (Setaria italica ) using Agrobacterium LBA4404 horboring the plasmid pBI121 was established. Factors affecting transformation efficiency were investigated including the genotype, explants, the density of bacteria, the duration for inoculation and co-cultivation, and the concentration of acetosyringone in the medium. The maximum transformation conditions were: the callus induced from inflorescence was used as explant; the duration for inoculation with Agrobacterium at low cell density was 30-40 min; for the co-cultivation , the suitable concentration of acetosyringone in the medium was 0.1 mmol/L, and the duration was 2 days.