农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
4期
44-46
,共3页
王恒强%孔庆军%任雪艳%占东霞%张海黎
王恆彊%孔慶軍%任雪豔%佔東霞%張海黎
왕항강%공경군%임설염%점동하%장해려
Chlorella vulgaris%Isolation method%DNA extraction method
[Objective] The aim of this study is to isolate Chlorella vulgaris (chlorella) and extract its genomic DNA. [Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples; the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method. [Result] The proper conditions for chlorella culture were as following: temperature 20-25 ℃, illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min; improved CTAB method was suitable for extracting genomic DNA from chlorella. [Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
