CAJ | 학술논문

从小鼠的睾丸中克隆了GSE(gonad-specific expression gene)基因,并对该基因进行序列分析和Southern、Northern杂交分析.核苷酸序列分析表明,GSE基因的ORF长度为745 bp,编码247个氨基酸,预测蛋白质分子量为27.6 kDa;Southern杂交证明GSE可能是单拷贝的基因;Northern杂交显示,GSE基因在小鼠体细胞组织(心脏、肝脏、肾脏、大脑、骨骼肌和脾脏)中没有检测到其表达,在睾丸中表达量很高,在卵巢中表达量较低.从推断出的氨基酸序列表明,GSE蛋白在细胞中可能是一个不带有信号肽的可溶性蛋白.
종소서적고환중극륭료GSE(gonad-specific expression gene)기인,병대해기인진행서렬분석화Southern、Northern잡교분석.핵감산서렬분석표명,GSE기인적ORF장도위745 bp,편마247개안기산,예측단백질분자량위27.6 kDa;Southern잡교증명GSE가능시단고패적기인;Northern잡교현시,GSE기인재소서체세포조직(심장、간장、신장、대뇌、골격기화비장)중몰유검측도기표체,재고환중표체량흔고,재란소중표체량교저.종추단출적안기산서렬표명,GSE단백재세포중가능시일개불대유신호태적가용성단백.
In this study, a novel gene termed GSE (gonad-specific expression gene) was identified. Sequence analysis of GSE revealed that the open reading frame (ORF) was 745 bp, which encodes a protein of 247 aa with a predicted molecular mass of 27.6 kDa. Southern blot analysis revealed GSE gene was a single copy fragment. Northern blot analysis showed that this gene was abundantly expressed in mouse testis and slightly expressed in the mouse ovary. The deduced amino acid sequence indicated that GSE protein might be a soluble protein in the cytoplasm without signal peptide.