CAJ | 학술논문

使用比色法测定水中微量三氯杀螨醇的浓度.首先用正己烷萃取浓缩水中的三氯杀螨醇,比较了加入不同量的去离子水,不同浓度、不同量的NaOH,不同的反应显色温度和反应时间以及吡啶用量等对体系吸光度的影响.得出最佳的显色条件:0.2ml去离子水,0.1ml质量浓度为45%的NaOH溶液,与2.0ml吡啶混合,于100℃水浴中反应3.0min,反应液在4000r/min离心2.0min,取出反应液在530nm处测定吸光度值.此方法的标准工作曲线相关系数为0.9914,方法的绝对检出限为0.2μg三氯杀螨醇.样品加标回收率为83.7%～114.1%,相对标准偏差小于10%.
사용비색법측정수중미량삼록살만순적농도.수선용정기완췌취농축수중적삼록살만순,비교료가입불동량적거리자수,불동농도、불동량적NaOH,불동적반응현색온도화반응시간이급필정용량등대체계흡광도적영향.득출최가적현색조건:0.2ml거리자수,0.1ml질량농도위45%적NaOH용액,여2.0ml필정혼합,우100℃수욕중반응3.0min,반응액재4000r/min리심2.0min,취출반응액재530nm처측정흡광도치.차방법적표준공작곡선상관계수위0.9914,방법적절대검출한위0.2μg삼록살만순.양품가표회수솔위83.7%～114.1%,상대표준편차소우10%.
The concentration of dicofol in water was determined using colorimetric determination method. Dicofol in water was first of all extracted and concentrated by n-hexane. Reaction conditions including dosage of de-ionized water, the dosage and concentration of NaOH, heating temperature and time, the dosage of pyridine were researched. The result showed that 0.2ml de-ionized water, 0.1ml 45% NaOH and 2.0ml pyridine, 3.0min heating time under 100℃ water bath are optimal conditions. Absorbency was determined at 530nm. the detection limit is 0.2μg dicofol by this method. Recoveries are between 83.7 and 114.1%, and RSD is less than 10%.