CAJ | 학술논문

目的:利用三亲本杂交方法将luxAB发光酶基因标记至荧光假单胞菌PF20001上,所获得的标记菌株PF20001-Lux能稳定发光.将该标记菌株制成微生物制剂,接种菜心进一步研究它在菜心根际的定殖动态和散布规律.方法:利用三亲本杂交方法将luxAB发光酶基因标记至荧光假单胞菌PF20001上,将该菌施与菜心生长土壤中,通过接合子发光检测及发光菌落的平板计数来分析荧光假单胞菌在菜心根际的定殖分布情况.结果:PF20001-Lux在根系周围的土壤中的有一定的定殖率,在根内主要定殖在3～4cm根内.PF20001-Lux在菜心种植后7d前就达到最高定殖水平达3.8×10~5 CFU/g,随后逐渐下降.结论:PF20001-lux在菜心根际具备良好的适应能力.
목적:이용삼친본잡교방법장luxAB발광매기인표기지형광가단포균PF20001상,소획득적표기균주PF20001-Lux능은정발광.장해표기균주제성미생물제제,접충채심진일보연구타재채심근제적정식동태화산포규률.방법:이용삼친본잡교방법장luxAB발광매기인표기지형광가단포균PF20001상,장해균시여채심생장토양중,통과접합자발광검측급발광균락적평판계수래분석형광가단포균재채심근제적정식분포정황.결과:PF20001-Lux재근계주위적토양중적유일정적정식솔,재근내주요정식재3～4cm근내.PF20001-Lux재채심충식후7d전취체도최고정식수평체3.8×10~5 CFU/g,수후축점하강.결론:PF20001-lux재채심근제구비량호적괄응능력.
Objective:By triparental mating, Pseudomonas flurosecens PF20001 strain marked by luxAB gene were successfully obtained,then the PF20001 - lux strain was made to microbial inoculant. After it was inoculated on rhizosphere of flowering Chinese cabbage, the colonizing dynamics and distribution of the luminescent bacteria PF20001 - lux in the rhizosphere of flowering Chinese cabbage plant were studied. Method:By triparental mating, Pseudomonas flurosecens PF20001 strain marked by luxAB gene then granted them to the rhizosphere of flowering Chinese cabbage by the method of decanal imaging and enumeration of luminescent colonies on agar media. Result: It showed that PF20001 - lux successfully colonized in the rhizosphere of flowering Chinese cabbage. The population which mainly colonized in the 3 -4 cm of root, reached to the highest level at day 7 post inoculation 3.8 × 10~5 CFU/g. The population of them trended to be stable and decreased to 0 at day 30 post inoculation. Conclusion: Pseudomonas flurosecens PF20001 have a high adaptation ability to colonize in the rhizosphere of flowering Chinese cabbage.