食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
3期
60-63
,共4页
徐响%董捷%丁小宇%杨佳林%孙丽萍
徐響%董捷%丁小宇%楊佳林%孫麗萍
서향%동첩%정소우%양가림%손려평
超临界二氧化碳萃取法%水蒸气蒸馏法%蜂胶%抑菌作用
超臨界二氧化碳萃取法%水蒸氣蒸餾法%蜂膠%抑菌作用
초림계이양화탄췌취법%수증기증류법%봉효%억균작용
supercritical CO_2 extraction%steam distillation extraction%propolis%antibacterial activity
采用水蒸气蒸馏法和超临界二氧化碳萃取法获得了蜂胶挥发油.GC-MS分析并鉴定出50种化合物,两种提取方法所得蜂胶挥发油在成分组成上存在显著差异.抑菌实验表明,两种提取方法获得的挥发油对金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌具有很强的抑制作用,而超临界法萃取的挥发油的抑菌效果强于水蒸气蒸馏法.
採用水蒸氣蒸餾法和超臨界二氧化碳萃取法穫得瞭蜂膠揮髮油.GC-MS分析併鑒定齣50種化閤物,兩種提取方法所得蜂膠揮髮油在成分組成上存在顯著差異.抑菌實驗錶明,兩種提取方法穫得的揮髮油對金黃色葡萄毬菌、枯草芽孢桿菌、銅綠假單胞菌具有很彊的抑製作用,而超臨界法萃取的揮髮油的抑菌效果彊于水蒸氣蒸餾法.
채용수증기증류법화초림계이양화탄췌취법획득료봉효휘발유.GC-MS분석병감정출50충화합물,량충제취방법소득봉효휘발유재성분조성상존재현저차이.억균실험표명,량충제취방법획득적휘발유대금황색포도구균、고초아포간균、동록가단포균구유흔강적억제작용,이초림계법췌취적휘발유적억균효과강우수증기증류법.
In this work,propolis essential oil was obtained by steam distillation extraction and supercritical CO2 extraction,respectively.Added together,50 components in resulting propolis essential oils were identified by GC-MS method.Propolis essential oils extracted by both methods exhibited strong inhibition effects against Staphylococcus aureus,Bacillus subtilis and Pseudomonas aeruginosa in vitro.Stronger inhibition effect of propolis essential oil extracted by supereritieal CO_2 extraction on the three species of bacteria than that of propolis essential oil extracted by steam distillation extraction was observed.
